مجله علوم پزشکی پارس

مقایسه اثر گیاه کرفس کوهی Kelussia odoratissima Mozaff بر بیان ژن SREBP-1C در رت های مبتلا به کبد چرب و رت های سالم

نویسندگان

واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران

چکیده

زمینه و هدف: تحقیقات نشان داده گیاه کرفس باعث کاهش سطح لیپوپروتئین با چگالی کم می­شود. با توجه به آمار فزاینده مبتلا به کبد چرب در این مطالعه برآن شدیم تا به بررسی اثر عصاره کرفس کوهی بر بیان ژن SREBP < /span>-1C در موش­های چاق مبتلا به کبد چرب بپردازیم.
مواد و روش کار: در تحقیق حاضر 24  سر رت به چهار گروه کنترل، کنترل منفی، تیمار و تیمار دوم تقسیم شدند. به دو گروه تیمار به ترتیب عصاره کرفس 400 و 800 میلی­لیتر بر کیلوگرم را به صورت گاواژ دریافت کردند. رت­ها با کلروفرم بیهوش شدند و پس از تشریح، بافت کبد رت­ها جمع آوری شد. سپس استخراج RNA و سنتز cDNA انجام شد. در نهایت بیان ژن SREBP-1C با استفاده از تکنیکRT- PCR   Real timeانجام شد و آنالیزهای مربوطه صورت گرفت.
نتایج: در تحقیق حاضر نتایج حاکی از آن بود که عصاره کرفس کوهی با دوز 800 میلی­گرم/کیلوگرم می­تواند باعث کاهش بیشتر بیان ژن SREBP-1C نسبت به دوز400 میلی­گرم/کیلوگرم در رت­های مبتلا به کبد چرب شود، این کاهش بیان از نظر آماری نسبت به گروه بیمار معنادار است (05/0p<). نتایج تست­های بیوشیمیایی بهبود کبد را در رت­های تیمار شده با دوز 800 میلی­گرم/کیلوگرم تایید می­کند.
بحث و نتیجه گیری: به نظر می­رسد عصاره کرفس کوهی به دلیل داشتن فلانوئید نارینجین سبب کاهش بیان ژن SREBP-1C می­شود که این ژن نقش مهمی در بیوژنز کلسترول، اسیدهای چرب و تری­گلیسیریدها دارد. در صورت تایید در مطالعات وسیع­تر می­تواند به عنوان یک هدف درمانی در بیماری کبد چرب مطرح باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The Effect of Kelussia odoratissima Mozaff Extract on SREBP-1C Gene Expression in Fatty Liver Rats and Healthy Rats

نویسندگان [English]

  • zahra haghshenas
  • noosha zia-jahromi

چکیده [English]

Background and Aim: Studies have shown that Kelussia lowers low-density lipoprotein levels. Due to the increasing number of patients with fatty liver in this study, we aimed to investigate the effect of Kelussia extract on the expression of SREBP-1C gene in obese mice with fatty liver.
 Materials & Methods: In the present study, 24 rats were divided into four groups: control, negative control, treatment and the second treatment. The two treatment groups received 400 and 800 ml/kg Kelussia extract by gavage, respectively. Rats were anesthetized with chloroform and after dissection, rat liver tissue was collected. Then RNA extraction and cDNA synthesis were performed. Finally, SREBP-1C gene expression was performed using RT-PCR Real time technique and relevant analyzes were performed.
Results: In the present study, the results showed that Kelussia extract at a dose of 800 mg/kg could further reduce the expression of SREBP-1C gene than a dose of 400 mg/kg in rats with fatty liver. It is statistically significant compared to the patient group (p <0.05). The results of biochemical tests confirm liver improvement in treated rats at a dose of 800 mg/kg.
Discussion and Conclusion: It seems that Kelussia extract reduces the expression of SREBP-1C gene due to its orange flavonoid, which plays an important role in the biogenesis of cholesterol, fatty acids and triglycerides. If approved in larger studies, it can be considered as a therapeutic target in fatty liver disease.

کلیدواژه‌ها [English]

  • Kelussia odoratissima Mozaff
  • SREBP-1C gene
  • Fatty liver
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مجله علوم پزشکی پارس
دوره 19، شماره 2 - شماره پیاپی 56
تیر 1400
صفحه 23-32