مقایسه اثر سافرانال و کروسین بر سطح سرمی آدیپونکتین، پروفایل لیپید و گلوکز در موش های صحرایی سالم و مبتلا به دیابت نوع یک

نویسنده
سید دامون صدوقی
دانشگاه آزاد اسلامی مشهد
10.52547/jmj.15.3.8
چکیده
زمینه و هدف: دیابت از شایع­ترین بیماری­های غدد درون­ریز است که با اختلال در متابولیسم گلوکز و چربی­ها همراه است. آدیپونکتین پروتئین اختصاصی مترشحه از بافت چربی است که می­تواند بر روند پیشرفت دیابت موثر باشد. با توجه به اثرات محافظتی کروسین به­عنوان یک ضد التهاب و آنتی­اکسیدانی قوی، هدف از این مطالعه تعیین اثر کروسین بر سطح سرمی آدیپونکتین، پروفایل لیپید و گلوکز در مدل موش­های صحرایی دیابتی نوع یک می­باشد.
مواد و روش­ها: در این مطالعه تجربی، 28 سر موش صحرایی نر نژاد ویستار به 4 گروه­ مساوی تقسیم شدند. گروه­های شاهد، شاهد دیابتی و دو گروه­­ دیابتی تحت تیمار با کروسین (غلظت­های 50 و 100 میلی­گرم بر کیلوگرم). دیابت در گروه­های شاهد دیابتی و دیابتی تحت تیمار، با یک­بار تزریق داخل صفاقی آلوکسان القاء شد. کروسین به مدت 25 روز به­صورت داخل صفاقی به گروه­های دیابتی تحت تیمار تزریق شد. به حیوانات گروه­های شاهد و شاهد دیابتی محلول سالین تزریق شد. در پایان دوره تیمار، سطح سرمی آدیپونکتین، LDL، HDL، تری گلیسرید، کلسترول تام و گلوکز توسط روش الایزا اندازه­گیری شد.
نتایج: در مقایسه با گروه شاهد دیابتی، سطح سرمی LDL، تری­گلیسرید، کلسترول تام و گلوکز در گروه­های دیابتی تیمار شده با غلظت­های 50 و 100 میلی­گرم بر کیلوگرم کروسین به­صورت وابسته به دوز کاهش، همچنین آدیپونکتین و HDL به طور معنی­داری افزایش یافت (05/0>p < /span>).
نتیجه­گیری: تجویز کروسین به­صورت وابسته به دوز از طریق افزایش سطح سرمی آدیپونکتین و کاهش قند خون در تنظیم متابولیسم چربی­ها نقش دارد.
کلمات کلیدی: دیابت، کروسین، آدیپونکتین، پروفایل لیپیدی، موش صحرایی
 
کلیدواژه‌ها
دیابت
کروسین
آدیپونکتین
پروفایل لیپیدی
موش صحرایی

عنوان مقاله English

Comparing the Effect of Safranal and Crocin on Serum Levels of Adiponectin, Lipid Profile and Glucose in Healthy and Type One Diabetic Rats

چکیده English

Background and Objective: Diabetes is the most common endocrine disease that is associated with impaired glucose and lipid metabolism. Adiponectin is a specific protein secreted from adipose tissue that can affect diabetes progression. Considering the protective effects of crocin, as a potent anti-inflammatory and powerful antioxidant, the aim of this study was to determine the effect of crocin on serum levels of adiponectin, lipid profile and glucose in type one diabetic rat’s model.

Materials and Methods: In this experimental study, 28 wistar male rats were allocated into 4 equal groups. Groups of control, diabetic control and two treated diabetic group with crocin (concentrations of 50 and 100 mg/kg). The diabetes in diabetic control and treated diabetic groups was induced using an intraperitoneal injection of alloxan. Crocin was intraperitoneally injected into treated diabetic groups for 25 days. Saline solution was injected to the animals of control and diabetic control groups. At the end of treatment period, the serum levels of adiponectin, LDL, HDL, triglyceride, total cholesterol and glucose was measured by ELISA.

Results: Compared to diabetic control group < /span>, serum levels of LDL, triglycerides, total cholesterol and glucose in diabetic groups treated with 50 and 100 mg/kg of crocin dose-dependent manner decreased, also adiponectin and HDL significantly increased (p<0.05).
Conclusion: Dose dependent administration of crocin by increases adiponectin and decreases blood glucose serum levels, plays a role in regulating lipid metabolism

کلیدواژه‌ها English

Key words: Diabetes
Crocin
Adiponectin
Lipid profile
Rat
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