مجله علوم پزشکی پارس

تغییرات بافت ریه ا ی گلبول های سفید خون تحت اثر تئولو ئن

نویسندگان

دانشگاه سیستان وبلوچستان

چکیده

چکیده

مقدمه

تولوئن (C7H8) ترکیبی آروماتیک وجزئی از ساختار بنزین بوده که به عنوان حلال شیمیایی در صنایع زیادی کاربرد دارد. این ماده اثرات نامطلوبی در محیط زیست داشته وبا توجه به استفاده ی روزافزون و عوارضِ گزارش شده ی آن ، هدف مطالعه ی حاضر، بررسی اثر تولوئن بربافت خون وریه است.



روش کار

30سرموش نربالغ نژادAlbino NMRIدر3گروه  ،شم،گروه تیمار Aکه به مدت 25روز هر روز یک نوبت  1/. سی سی تولوئن + سرم فیزیولوژیک ( 35./. تولوئن + 65./. سرم فیزیولوژیک ) باعیار mg/kg 1700وزن موش وگروه تیمار B با شرایط مشابه که عیار mg/kg  1000تولوئن را دریافت کردندمورد مطالعه قرار گرفتند. .پس ازوزن­کشی،بیهوشی وخون­گیری،پارامترهای مورد نظربافت ریه و خون، با نرم افزارموتیک اندازه گیری ومطالعات ماکروسکوپی و میکروسکوپی از طریق لامهای تهیه شده،انجام شد



یافته ها

در گروه تیمارA ،درصد نوتروفیل ها کاهش و تعداد لنفوسیتهای دژنره و انواع دیگر گلبولهای سفید افزایش یافت. بافت ریه،در میانگین ضخامت دیواره برونش ،قطر کلی برونشیول انتهایی ،برونشیول تنفسی ، ضخامت سپتای آلوئول و  تعدادسلولهای پنوموسیتهای 2افزایش ودر پنوموسیت 1کاهش نشان داد،که درتمامی پارامترهای ذکر شده، ارتباط گروه Aبا گروهB وشم وارتباط دوگروه Aو B نسبت به یکدیگر معنی دار بود( 5 0/0>P < /span> )

بحث و نتیجه گیری

تولوئن با تحریک مراکز خونساز، باعث تغییرات درتعداد ودرصد انواع گلبولهای سفیدوباافزایش درضخامت وقطر پارامترهای ریوی، التهاب وتنگی آلوئولها، موجب ضعف سیستم تنفسی وکاهش عملکرد آن شد،که این اثرات وابسته به دوز بود ودر عیار بالاتر تولوئن اثرات آسیب رسان بیشتری رانشان داد.

کلیدواژه‌ها

عنوان مقاله [English]

The toxic effects of Toluene on the the lungs, spleen and blood parameters in mice

نویسندگان [English]

  • Adeli HamidRaza
  • Kobra Zarei
  • Nasim Naeimi
  • Maryam Alamdar

چکیده [English]

Introduction

Toluene (C7H8) is an aromatic compound and part of the structure of petroleum, which is mainly used as a chemical solvent in the industry. Being harmful to the environment and with respect to the increasingly use and side effects, this substance and its impact on blood and lunges tissues, have been studied on in this study.

Material and Methods

30 mature Albino NMRI mice have been experimented in three groups of Shem, Group-A, which has been injected with the 0.1 cc Toluene solution (35% Toluene and 65% physiologic solution) with the purity of 1700 mg/kg for 25 days and Group-B with the same weight of the mice and the same situation except the purity which has been changed to 1000 mg/kg. After weighting, anesthesia and taking blood samples, relative parameters of blood and lung tissues were considered, measured and studied in the scope of micro and macro.

Reasalt

In the Group-A the percentage of Neutrophil have been reduced and the number of degenerated Lymphocytes and other leukocytes have been enhanced. In lungs' tissues the mean thickness of bronchi walls, the overall diameter of bronchioles end, breathing bronchiole, and the thickness of the Alveoli septa in group-A have shown improvements, also pneumocystis1 cells and pneumocystis 2 cells have been raised and reduced respectively. At all the mentioned parameters there are meaningful relations between A and B and shame, with a P<0.05.

Discussion

By stimulating Blood makingcenters, Toluene causes changes in the number and the percentages of leukocytes and by raising the thickness and the diameter of parameters of lungs, causes breathing weakness and the reduction of its performance. These impacts are dependent to the dosage of the Toluene of which the high purity has shown much more damage.

کلیدواژه‌ها [English]

  • Toluene
  • Hematology
  • Lung Tissue
  • Albino NMRI Male white mice
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مجله علوم پزشکی پارس
دوره 14، شماره 1 - شماره پیاپی 35
فروردین 1395
صفحه 27-37