تأثیر عصاره هیدروالکلی ریشه‌ی گیاه ثعلب (Dactylorhiza maculata) بر برخی شاخص‌های خونی و پروتئین‌های سرم در موش‌های صحرایی سالم

نوع مقاله : مقاله پژوهشی

نویسندگان
1 مرکز تحقیقات بیماری های غیر واگیر،دانشگاه علوم پزشکی جهرم، جهرم، ایران
2 کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی جهرم، جهرم، ایران
3 کمیته تحقیقات دانشجویی، دانشگاه علوم پزشکی جهرم، جهرم، ایران.
4 متخصص عفونی، استادیار فلوشیپ پیشگیری و کنترل عفونت (IPC)، بخش بیماری‌های عفونی، گروه داخلی، دانشگاه علوم پزشکی شیراز، شیراز، ایران
5 مرکز تحقیقات سالمندی شیراز، دانشگاه علوم پزشکی شیراز، شیراز، ایران
چکیده
مقدمه: اعضای خانواده ثعلب (Orchidaceae) به‌دلیل اثرات تعدیل‌کننده سیستم ایمنی و هماتولوژیک شناخته شده‌اند. این مطالعه با هدف ارزیابی تأثیر عصاره هیدروالکلی ریشه ثعلب (Dactylorhiza maculata) بر سلول‌های خون محیطی و پروتئین‌های سرم در موش‌های صحرایی سالم انجام شد.
روش‌ها: چهل و هشت موش صحرایی ویستار نر بالغ (۲۰۰–۱۸۰ گرم) به‌طور تصادفی به شش گروه تقسیم شدند: گروه کنترل، شاهد (دریافت‌کننده حلال) و چهار گروه تیمار شده با عصاره (دوزهای 80، 160، 320 و 640 میلی‌گرم بر کیلوگرم به مدت ۲۸ روز از طریق گاواژ). روز بیست‌ونهم، نمونه‌های خون برای سنجش پارامترهای هماتولوژیک شامل گلبول قرمز، گلبول سفید، هموگلوبین، هماتوکریت، پلاکت، اندیس‌های گلبول قرمز و شمارش افتراقی گلبول سفید جمع‌آوری شد. سطوح پروتئین‌های سرم نیز اندازه‌گیری گردید.
یافته‌ها: عصاره ریشه ثعلب در دوز ۶۴۰ میلی‌گرم بر کیلوگرم به‌طور معنی‌داری هماتوکریت، غلظت هموگلوبین و شمارش گلبول‌های قرمز را افزایش داد (P<0.05). عصاره در دوزهای ۳۲۰ و ۶۴۰ میلی‌گرم بر کیلوگرم باعث افزایش معنی‌دار MCV، MCH و MCHC گردید. تعداد گلبول‌های سفید تحت تأثیر قرار نگرفت، اما تغییرات معنی‌داری در درصد نوتروفیل‌ها و لنفوسیت‌ها مشاهده شد. شمارش پلاکت‌ها و سطوح پروتئین‌های سرم تغییر معنی‌داری نشان ندادند.
نتیجه‌گیری: یافته‌ها نشان داد که عصاره ریشه ثعلب، به‌ویژه در دوزهای بالا، احتمالاً موجب افزایش اریتروپوئزیس و تعدیل توزیع سلول‌های ایمنی می‌شود، بدون آن‌که تأثیر چشمگیری بر شمارش کل گلبول‌های سفید یا سطوح پروتئین‌های سرم داشته باشد. با توجه به نبود آنالیز فیتوشیمیایی در مطالعه حاضر، هرگونه نسبت دادن این اثرات به ترکیبات خاص مانند فلاونوئیدها و پلی‌ساکاریدها تنها در سطح فرضیه مطرح است.
کلیدواژه‌ها

عنوان مقاله English

Effect of Dactylorhiza maculata (Orchidaceae) root hydroalcoholic extract on some hematological Parameters and serum Proteins in healthy Rats

نویسندگان English

Hossein Kargar Jahromi 1
Mohammad Aref Bagherzadeh 2
Hoda Haghshenas 3
Rahim Raoofi 4
Bahareh Ebrahimi 5
1 Research center for non-Communicable Disease, Jahrom University of Medical Sciences, Jahrom, Iran.
2 Student Research Committee,Jahrom University of Medical Sciences,Jahrom, Iran
3 Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran
4 Infectious Specialist, Assistant Professor of Infection Prevention Control Fellowship (IPC). Division of Infectious Disease, Department of Internal Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
5 Shiraz Geriatric Research Center, Shiraz University of Medical Science, Shiraz, Iran
چکیده English

Introduction: Members of the orchid family (Orchidaceae) are known for their immunomodulatory and hematological effects. This study aimed to evaluate the effect of hydroalcoholic extract of Dactylorhiza maculata (DM) root on peripheral blood cells and serum proteins in healthy rats.
Methods: Forty-eight adult male Wistar rats (180–200 g) were randomly divided into six groups: control, sham (solvent), and four extract-treated groups (doses of 80, 160, 320, and 640 mg/kg for 28 days by gavage). On the 29th day, blood samples were collected for hematological parameters including Red blood cells (RBC), white blood cells (WBC), hemoglobin, hematocrit, platelet count, red blood cell indices, and WBC differential count. Serum protein levels were also measured.
Results: DM root extract at a dose of 640 mg/kg significantly increased hematocrit, hemoglobin concentration, and red blood cell count (P<0.05). The extract at doses of 320 and 640 mg/kg significantly increased Mean corpuscular volume, mean corpuscular hemoglobin, and mean corpuscular hemoglobin concentration. Total WBC count was not affected, but significant changes were observed in the percentage of neutrophils and lymphocytes. Platelet counts and serum protein levels did not show significant changes.
Conclusion: The findings indicated that DM root extract, especially at high doses, may increase erythropoiesis and modulate immune cell distribution, without significantly affecting total WBC count or serum protein levels. Given the lack of phytochemical analysis in the present study, definitive confirmation of these effects and identification of the responsible compounds require further studies.

کلیدواژه‌ها English

Dactylorhiza maculata
Orchidaceae
Hematology
Serum proteins
RBC
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