اثر مهاری اینترفرون بتا در آزاد سازی میکروپارتیکل های مشتق از سلول های اندوتلیال در بیماران مبتلا به مولتیپل اسکروزیس

نویسندگان
اکبر هاشمی طیر
مریم جلالی
مریم کامروان
مرکز تحقیقات بیماری های غیر واگیر، دانشگاه علوم پزشکی جهرم، جهرم، ایران
10.22034/pjms.2022.700533
چکیده
مقدمه: افزایش مقدار میکروپارتیکل ها (MPs) در بسیاری از بیماری های خودایمنی همچون مولتیپل اسکلروزیس (MS) دیده شده است. در MS به دنبال فعال شدن لنفوسیت ها و تولید سایتوکاین های التهابی، سلول های اندوتلیال از غشای خود MPs آزاد می کنند. هدف از این مطالعه بررسی اثر مهاری داروی اینترفرون بتا ((INFβ) بر آزادسازی MPs مشتق شده از سلول های اندوتلیال (EnMPs) بود.
روش کار: ۲۰ نفر از بیماران مبتلا به MS که بیماری آن ها به تازگی تشخیص داده شده بود وارد مطالعه شدند. نمونه خون، در دو مرحله قبل و سه ماه بعد از درمان با اینترفرون بتا از بیماران گرفته شد. از فلوسیتومتری برای شمارش و تعیین فنوتیپ MPs و برای نشاندار کردن EnMPs  از anti-CD51 و anti-CD31 استفاده شد. همچنین در هر دو مرحله، MRI بیماران از نظر تعداد پلاک های مغزی مورد بررسی قرار گرفت. داده ها با کمک نرم افزار آماری SPSS نسخه 23 تجزیه و تحلیل شدند.
یافته ها: میانگین سنی بیماران، 8.3 ± 31.3 سال بود. نتایج این مطالعه، کاهش ۴۰ درصدی مقدار CD31+MPs و ۳۲ درصدی سطح CD51+MPs به دنبال درمان با داروی اینترفرون بتا نشان داد که از نظر آماری معنادار بود (p<0.001). در این مطالعه، ارتباط مثبت و معناداری بین تعداد EnMPs با تعداد پلاک های مغزی  (p=0.067)، سن (p=0.058) و جنسیت بیماران (p=0.061) مشاهده نشد.
نتیجه گیری: اینترفرون بتا اثر مهاری بر آزادسازی EnMPs از سلول‌های اندوتلیال دارد و نشان می‌دهد که EnMPs احتمالاً می‌توانند حساس برای پایش پیشرفت بیماری و اثربخشی درمان استفاده شوند.
کلیدواژه‌ها
مولتیپل اسکلروزیس
اینترفرون بتا
میکروپارتیکل مشتق شده از اندوتلیال

عنوان مقاله English

Inhibitory effect of the interferon-beta on the release of endothelial cell derived microparticles in patients with multiple sclerosis

نویسندگان English

Akbar Hashemi Tayer
Maryam Jalali
Maryam Kamravan
Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran
چکیده English

Introduction: Increased levels of microparticles (MPs) have been reported in many autoimmune diseases such as multiple sclerosis (MS). In MS, endothelial cells release MPs from their membranes following the activation of lymphocytes and the production of inflammatory cytokines. The aim of this study was to investigate the inhibitory effect of interferon beta (INFβ) on the release of endothelial cell-derived MPs (EnMPs).
Material and Methods: Twenty newly diagnosed MS patients were included in this study. Blood samples were taken from
patients in two stages before and three months after INFβ treatment. Flow cytometry was used to count and determine the phenotype of MPs. Anti-CD51 and anti-CD31 were used to tag EnMPs. In both stages, patients' MRI was also evaluated for the number of brain plaques. Data were analyzed using SPSS-23 statistical software.
Result: The mean age of patients was 31.3 ± 8.3 years. The results of this study showed a 40% decrease in the level of CD31+ MPs and a 32% decrease in the level of CD51+ MPs following treatment with INFβ, which was statistically significant (p <0.001). In this study, there was no positive and significant relationship between the number of EnMPs and the number of brain plaques (p = 0.067), age (p = 0.058) and sex of patients (p = 0.061).
Conclusion: INFβ has an inhibitory effect on the release of EnMPs from endothelial cells and suggest that EnMPs can probably be used as sensitive biomarkers to monitor disease progression and treatment effectiveness.

کلیدواژه‌ها English

Multiple Sclerosis
Interferon Beta
Endothelial Cell-Derived Microparticles
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