1. Edrisiian G, Rezaeian M, Mohebali M, Keshavarz H. Medical Protozoology. 1st, editor: Tehran University of Medical Sciences; 2008(Text in Persian). 2. Visvesvra GS, Moura H, Schuster FL. Pathogenic and opportunistic free-living amoebae: Acanthamoeba spp. Balamuthia mandrillaris, Naegleria fowleri, and Sappinia diploidea.FEMS Immunol Med Microbiol 2007; 50(1):1-26. 3. Hooshyar H, Rezaian M. Amoebas .2nd Edition. Tehran University of Medical Sciences; 2016(Text in Persian). 4. Marciano Cabral F, Cabral G. Acanthamoeba spp. as Agents of Disease in Humans. Clin Microbiol Rev 2003; 16(2): 273-307. 5. Fritsche TR, Gautom RK, Seyedirashti S, Bergeron DL, Lindquist TD. Occurrence of bacterial endosymbionts in Acanthamoeba spp. isolated from corneal and environmental specimens and contact lenses. J Clin Microbiol 1993; 31(5):1122-6. 6. Kuiper MW, Wullings BA, Akkermans AD, BeumerRR, KooijDV. Intracellular proliferation of Legionella pneumophila in Hartmanella vermiformis in aquatic biofilms grown on plasticized polyvinyl chloride.Appl Environ Microbiol 2004; 70:6826-6833. 7. Dey R, Rieger A, Banting G, Ashbolt NJ. Role of amoebae for survival and recovery of ‘non-culturable’Helicobacter pylori cells in aquatic environments. FEMS Microbiol Ecol 2020; 96(10):fiaa182. 8. Medie FM, Salah IB, Henrissat B, Raoult D, Drancourt M. Mycobacterium tuberculosis complex mycobacteria as amoeba-resistant organisms. PLoS One. 2011; 6(6):e20499. 9. Hassan A, Farouk H, Hassanein F, Abdul-Ghani R, Abdelhady AH. Acanthamoeba contamination of hemodialysis and dental units in Alexandria, Egypt: A neglected potential source of infection. J Infect Public Health. 2012; 5(4):304-10. https://doi.org/10.1016/j.jiph.2012.06.001 PMID:23021653. 10. Eftekhari Kenzerki R, Solhjoo K, Babaei Z, Rezanezhad H, Abolghazi A .Detection of Naegleria and Identification of Naegleria australiensis from Swimming pools in Kerman Province . Pars Journal of Medical Sciences, 2020; 18(2):19-25. 11. Castro-Artavia E, Retana-Moreira L, Lorenzo-Morales J, Abrahams-Sandi E. Potentially pathogenic Acanthamoeba genotype T4 isolated from dental units and emergency combination showers. MemInst Oswaldo Cruz 2017; 112(12):817-21. 12. Golestani MH, Rasti S, Hooshyar H, Delavari M, Mousavi SGA, et al. Molecular Identification and Genotyping of Acanthamoeba Isolated from Environmental Sources in Kashan, Central Iran. Jundishapur J Microbiol 2018; 11(4) e55582. 13. Lasjerdi Z, Niyyati M, Haghighi A, Shahabi S, Biderouni FT, Taghipour N, et al. Potentially pathogenic free-living amoebae isolated from hospital wards with immunodeficient patients in Tehran, Iran. Parasitol Res 2011; 109(3):575-80.7 14. Martínez-Girón R, Esteban JG, Ribas A, Doganci L. Protozoa in respiratory pathology: a review. Eur Respir J 2008; 32(5):1354-70. 15. Duarte AG, Sattar F, Granwehr B, Aronson JF, Wang Z, Lick S. Disseminated Acanthamoebiasis after Lung Transplantation.J Heart Lung Transplant 2006; 25(2):237-40. [16] Khan NA. Pathogenesis of Acanthamoeba infections. Microb Pathogen 2003; 34:277–85. 17. Ghamilouie MM, Valadkhani Z, Rahimi F, Khoshzaban F, Aghighi Z, Hassan N. Isolation and Genotyping of Acanthamoeba Strains from Corneal Scraps. IrJO 2014; 26(2):97-101. 18. Kalra SK, Sharma P, Shyam K, Tejan N, Ghoshal U. Acanthamoeba and its pathogenic role in granulomatous amebic encephalitis. Exp. Parasitol 2020; 208:107788. 19. Schuster FL, Visvesvara GS. Free-living amoebae as opportunistic and non-opportunistic pathogens of humans and animals. Int J Parasitol 2004; 34: 1001–27. 20. Zabel RW. Acanthamoeba keratitis. Increasing the index of suspicion. Can J Ophthalmol 1995; 30:33-34. 21. Alizadeh M, Khademvatan SH, Spotin A. Isolation and molecular characterization of Acanthamoeba isolated from immunocompromised patients hospitalized in Tabriz hospitals. 4th International Congress and the 11th National Congress of Parasitology and Parasitic Diseases of Iran (NICOPA11) Urmia-Iran Oct, 2019. 22. Memari F, Niyyati M Haghighi A ,Seyyed Tabaei S.J, Lasjerdi Z. Occurrence of pathogenic Acanthamoeba genotypes in nasal swabs of cancer patients in Iran . Parasitol Res 2015 114:1907–1912 .DOI 10.1007/s00436-015-4378-2 23. Arab-Mazar Z, Niyyati M, Lasjerdi Z, Spotin A, Darzam IA, Gachkar L. Isolation, identification, and phylogenetic analysis of potentially pathogenic free-living amoebae isolated from nasal and oral mucosa of HIV/AIDS patients in Iran. Parasitol Res 2019 Oct; 118(10):3061-6. 24. Pezeshki A, Haniloo A, Mahmoodzadeh A, Farahmandian P. Morphological Identification of Acanthamoeba spp. Isolated from Malignant Patients from Zanjan. J Hum Environ Health Promot.2018; 4(2):71-4. 25. Golestani M H, Rasti S, Hooshyar H, Delavari M, Mousavi G A, Iranshahi L. Isolation of Free-living amoeba and Molecular Characterization of Acanthamoeba From Stagnant Water, Kashan, Iran. Iran J Med Microbiol. 2018; 12 (2): 125-132 26. Mostafaei G, Bidgoli MS, Rasti S, Moosavi SG, Iranshahi L. Genotyping of acanthamoeba in rural drinking water sources in Kashan and Aran-Bidgol, Iran. J Mazandaran Univ Med Sci.2019; 28(169):130-9. 27. Tilak R, Singh RG, Wani IA, Parekh A, Prakash J, Usha U. An unusual case of acanthamoeba peritonitis in a malnourished patient on continuous ambulatory peritoneal dialysis (CAPD). J Infect Dev Ctries 2008; 2(2):146-48 28. Galaraza C, Ramos W, Gutierrez EL, Ronceros G, Teran M, et al. Cutaneous acanthamebiasis infection in immunocompetent and immunocompetent patien. Int J Dermatol 2009; 48(12):1324-29. 29. Petry F, Torzewski M, Bohl J, Wilhelm-Schwenkmezger T, Scheid P, Walochnik J, Michel R, Zöller L, Werhahn KJ, Bhakdi S, Lackner KJ (2006) Early diagnosis of Acanthamoeba infection during routine cytological examination of cerebrospinal fluid. J Clin Microbiol 44(5):1903–1904 .