مجله علوم پزشکی پارس

پاسخ miR-133a و برخی شاخص های هایپرتروفی قلبی به یک دوره تمرین استقامتی

نویسندگان

1 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی، واحد آیت اله آملی، دانشگاه آزاد اسلامی،آمل، ایران

2 گروه فیزیولوژی ورزشی، دانشکده تربیت بدنی و علوم ورزشی،واحد آیت اله آملی، دانشگاه آزاد اسلامی،آمل، ایران

3 گروه فیزیولوژی ورزشی،دانشکده تربیت بدنی و علوم ورزشی،واحددامغان،دانشگاه آزاد اسلامی،دامغان، ایران

چکیده

​مقدمه: اثر فعالیت ورزشی بر بیان ژن های اثرگذار بر هایپرتروفی قلبی مشخص نیست. شواهد نشان می دهد که برخی از miRNA ها در بهبود عملکرد قلب نقشی مهمی ایفا می ­کنند. هدف از این پژوهش، پاسخ ژن miR-133a و برخی شاخص های هایپرتروفی قلبی به یک دوره تمرین استقامتی بود.
روش کار: در این مطالعه تجربی، 10 سر موش صحرایی نر چهار تا شش هفته ای نژاد ویستار به طور تصادفی در دو گروه کنترل و تمرین قرار گرفتند. تمرین استقامتی، پنج روز در هفته با سرعت 25 متر بر دقیقه و شیب صفر درجه به مدت دوازده هفته اجرا شد. میزان بیان ژن­ های miR-133a ،  IGF-1 و IGF-R به روش Real Time PCR اندازه­ گیری شد. برای تجزیه و تحلیل داده ­ها از آزمون t مستقل در سطح معناداری آماری 0.05>P  استفاده شد.
یافته­ ها: نتایج نشان داد که پس از دوازده هفته تمرین هوازی بیان ژن miR-133a و IGF-1 در گروه تمرین نسبت به گروه­ کنترل به طور معناداری بالاتر بود (p=0.001). همچنین، بعد از دوازده هفته تمرین هوازی تفاوت معناداری در بیان ژن IGF-R  بین دو گروه مشاهده نشد (p>0.05).
نتیجه‌گیری: با توجه به یافته های پژوهش حاضر، دوازده هفته تمرین هوازی احتمالا می تواند فعالیت های حفاظت قلبی را از طریق شاخص های هایپرتروفی قلبی افزایش دهد.  
 
 

کلیدواژه‌ها

عنوان مقاله [English]

The Response of miR-133a and Some Markers of Cardiac Hypertrophy to Endurance Training

نویسندگان [English]

  • Susan Shahin jahromi 1
  • Asieh Abbassi daloii 2
  • Alireza Barari 2
  • Ayyoub Saeidi 3

1 Department of Physical Education and Sport Science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

2 Department of Physical Education and Sport Science, Ayatollah Amoli Branch, Islamic Azad University, Amol, Iran

3 Department of Physical Education and Sport Science, Damghan Branch, Islamic Azad University, Damghan, Iran

چکیده [English]

Introduction: The effect of exercise on the expression of genes affecting cardiac hypertrophy is unclear. Evidence
suggests that some miRNAs play an important role in improving heart function. The aim of this study was to evaluate the response of miR-133a and some markers of cardiac hypertrophy to endurance training.
Materials and Methods: To implementation of this experimental research, 10 male Wistar Rats (4-6 weeks old) randomly were divided into 2 groups control and endurance training. Endurance training performed 5 days a week at a
speed of 25 m/min and a gradient of zero degrees for 12 weeks. The expression of miR-133a, IGF-1 and IGF-R genes were measured by Real Time PCR. Data were analyzed by independent t-test at the P<0.05.
Results: The results showed that after 12 weeks of aerobic exercise the expression of miR-133a and IGF-1 gene
was significantly higher in the exercise group than in the control group (P=0.001). Also, after 12 weeks of aerobic training, no significant difference was observed in IGF-R gene expression between the two groups (P >0.05).
Conclusion:
According to the findings of the present study, twelve weeks of aerobic exercise may possibly increase
cardiac protection activities through cardiac hypertrophy indices

کلیدواژه‌ها [English]

  • Exercise
  • Cardiac Hypertrophy
  • miR-133a
  • IGF-1
  • IGF-R
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مجله علوم پزشکی پارس
دوره 18، شماره 1 - شماره پیاپی 51
فروردین 1399
صفحه 50-57