ارتباط پلی‌مورفیسم ژن Beta fibrinogen-455 G/A(rs1800790) با سقط مکرر در زنان ایرانی

نویسندگان
محمد شکرزاده 1
عباس محمدپور 2
زینب حیدری 3
1 استاد، مرکز تحقیقات علوم دارویی،گروه سم شناسی و فارماکولوژی، دانشکده داروسازی، دانشگاه علوم پزشکی مازندران، ساری، ایران
2 دکترای سلولی و مولکولی، مرکز تحقیقات علوم دارویی،گروه سم شناسی و فارماکولوژی، دانشکده داروسازی،دانشگاه علوم پزشکی مازندران، ساری، ایران
3 دانشجوی کارشناسی ارشد، گروه ژنتیک، موسسه آموزش عالی سنا، ساری، ایران
10.52547/jmj.17.1.56
چکیده
زمینه و هدف :  سقط مکرر یک اختلال ژنتیکی با علل مختلف است که طبق تعریف سازمان بهداشت جهانی، به سه بار یا بیشتر از دست دادن حاملگی گفته می شود. ناهنجاری های ژنتیکی یکی از علل اصلی سقط مکرر است. هدف پژوهش حاضر بررسی ارتباط پلی مورفیسم ژن Beta fibrinogen (rs1800790) و سقط مکرر در زنان ایرانی بود.
روش بررسی : در این مطالعه ی مورد– شاهدی101 زن دچار سقط مکرر (41-19 سال) و 50 زن بدون سابقه سقط (57-23 سال) (کنترل) شرکت داشتند. پنج میلی لیتر از خون این افراد در لوله های حاوی EDTA در بیمارستان امام خمینی شهر ساری طی سال های 1397-1396 جمع آوری شد. DNA ژنومی با روش salting out استخراج و به روش PCR-RFLP بررسی ژنوتیپی پلی مورفیسم انجام شد. داده ها با استفاده از نرم افزار Medcalc نسخه 15 تحلیل آماری شدند.
یافته ها : شیوع فراوانی ژنوتیپی GG ,GA ,AA ژن FGB به ترتیب در گروه بیمار 5.94% ، 29.7% ، 64.36%، و در گروه کنترل 2% ، 50% ، 48% بود(p=0.040). درصد فراوانی اللی G و A به ترتیب در گروه بیمار 79% و 21% و در گروه کنترل 73% و 27% بود (p=0.322). شواهد نشان داد ژن موتانت AA ، خطر سقط مکرر را افزایش نمی دهد و ارتباط معناداری وجود ندارد (p=0.472). اما هتروزیگوت GA  نسبت به ژنوتیپ مرجع GG خطر سقط مکرر را کاهش می دهد (OR=0.44, 95%CI:0.22-0.90)(p=0.024).
نتیجه گیری :  نتایج نشان داد که پلی مورفیسم  rs1800790 ژن FGB، خطر بروز سقط مکرر را افزایش نمی دهد.
کلیدواژه‌ها
پلی مورفیسم rs1800790
سقط مکرر
Beta fibrinogen-455 G/A

عنوان مقاله English

The association of beta fibrinogen-455 G/A (rs1800790) gene polymorphism with recurrent abortion in Iranian women

نویسندگان English

Mohammad Shokrzadeh 1
Abbas Mohammadpour 2
Zeinab Heidari 3
1 Professor, pharmacutical research center, department of Toxicology and pharmacology, faculty of pharmacy, mazandaran university of medical sciences, sari, Iran
2 Ph.D cell &molecular biology, pharmacutical research center, department of toxicology and pharmacology, faculty of pharmacy, mazandaran university of medical sciences, sari, Iran
3 Graduate student of genetics, Sana Institute of Higher Education, Sari, Iran
چکیده English

Introduction: Recurrent Abortion is a genetic disorder with different etiologies, as defined by the World Health
Organization (WHO), three or more times consecutive pregnancy losses. Genetic anomalies are one of the main etiologies of recurrent abortion. The purpose of the study was to investigate the association between beta fibrinogen (rs1800790) gene polymorphism and recurrent abortion in Iranian women.
Materials and Methods: In this case-control study, 101 women with recurrent abortion (19-41 years) and 50 women with no history of abortion (23-57 years) (controls) were present. Five milliliters of their blood was collected in EDTA tubes in Imam Khomeini hospital, Sari, Iran, during 2017-2018. Genomic DNA was extracted using salting-out method and genotypic study of polymorphism was performed using PCR-RFLP method. Statistical analysis was performed using Medcalc software version 15.
Results: Relative frequency of AA, GA, GG genotypes for FGB in patient group were 5.94%, 29.7% and 64.36% and in control group were 2%, 50%, 48% respectively (p= 0.040). The percentage of G and A alleles was 79% and 21% in patient and 73% and 27% in control groups, respectively (p= 0.322). Evidence indicated the AA mutant gene does not increase the risk of recurrent abortion and there is no significant association (p= 0.472). But GA heterozygote relative to GG reference genotype decrease the risk of recurrent abortion (OR= 0.44, 95%CI: 0.22-0.90, P= 0.024).
Conclusion: The results showed that rs1800790 FGB gene polymorphism did not increase the risk of recurrent
abortion.

کلیدواژه‌ها English

Rs1800790
Recurrent abortion
Beta Fibrinogen- 455 G/A
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