تجویز همزمان اسیدچرب لینولنیک و لینولئیک، لیپوتوکسیسیتی را در سلول های قلبی کاهش داد.

نویسندگان

وزارت بهداشت درمان وآموزش پزشکی

چکیده

مقدمه: نوع، شدت و مدت ورزش تعیین‌کننده میزان اثر ورزش بر روی شاخص‌های هماتولوژیکی است. هدف مطالعه حاضر تعیین اثر شدت ورزش بر پلاکت و شاخص­های هماتولوژیکی دختران فوتسالیست دانشگاهی می باشد.
روش کار : یازده دختر فوتسالیست دانشگاه جهرم که حداقل 6 ماه سابقه­ی تمرین فوتسال داشتند به صورت داوطلبانه در این مطالعه نیمه تجربی شرکت کردند. آزمودنی­ها در هفته­ی اول یک جلسه با شدت 65% ضربان قلب بیشینه و در هفته دوم یک جلسه با شدت 80% ضربان قلب بیشینه، به مدت 30 دقیقه در پیست دو و میدانی دویدند. نمونه­های خونی، قبل و بلافاصله بعد از هر جلسه تمرین از آزمودنی­ها گرفته شد.
یافته­ها: نتایج نشان داد که پس از فعالیت با شدت متوسط، تعداد پلاکت‌ها، درصد هماتوکریت و متوسط حجم سلول‌های قرمز افزایش معنی­داری دارد (05/0p <). همچنین پس از فعالیت با شدت زیاد، تعداد پلاکت‌ها و متوسط حجم سلول‌های قرمز افزایش معنی­داری (05/0p <) و میزان هموگلوبین و غلظت متوسط هموگلوبین سلول‌های قرمز کاهش معنی­داری نشان داد (05/0p <). تفاوت معنی‌داری در مقادیر هموگلوبین، میانگین هموگلوبین سلول‌های قرمز و غلظت متوسط هموگلوبین سلول‌های قرمز بین فعالیت با شدت متوسط و زیاد وجود داشت (05/0p <).
بحث و نتیجه‌گیری: بر اساس یافته­ های مطالعه ­ی حاضر فعالیت حاد با شدت­های مختلف از نوع دویدن می­تواند اثر معنی­داری بر روی برخی پارامترهای خونی دختران فوتسالیست دانشگاهی داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Coadministration of linolenic acid and linoleic acid decreased lipotoxicity in cardiomyocytes.

نویسندگان [English]

  • Elham Zadeh Hashem
  • mina khodadadi
  • lachin mousavi

چکیده [English]

Background: Deleterious effects of lipid accumulation in non-adipose tissues are known as lipotoxicity. The prevalence of cardiovascular disease due to lipotoxicity has been dramatically increased worldwide. Based on available reports, palmitic acid induced lipotoxicity in several cell type like cardiomyocyte and hepatocytes while oleic acid could prevent the lipotoxicity in rat hepatocytes because Unsaturated fatty acids may counteract the lipotoxicity associated with saturated fatty acids. This study aimed to investigate the effects of combination of linoleic acid with linoleic acid on the induced lipotoxicity by palmitic acid in adult rat cardiomyocyte.
Methods: Ventricular heart muscle cells were isolated and seeded into 24-well-plates. Cells were treated with palmitic (0.5 mM), linoleic acid (0.25 mM)+linolenic acid (0.5 mM), palmitic+ linoleic acid+linolenic acid (0.5 mM+0.25 mM+0.5 mM) or left as control. Triacylglycerol (TAG), diacylglycerol (DAG), DNA fragmentation and percent of viability were measured at 24, 48 hours.
Findings: Result showed that linoleic acid+linolenic acid decreased DAG and DNA fragmentation and increased TAG and cell viability when co-administrated with palmitic in comparison with palmitic alone (p<0.05).
Conclusion: In conclusion, combonation of linoleic+linolenic acid would improve the lipotoxicity indices of palmitic acid in primary adult rat cardiomyocytes culture.  

کلیدواژه‌ها [English]

  • linolenic acid
  • Linoleic acid
  • lipotoxicity
  • palmitic acid
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