شناسایی ژن های بتالاکتاماز در اشرشیا کلی جداشده از زخم پای دیابتی به روش Multiplex PCR در شهرستان جهرم

نویسندگان

دانشگاه علوم پزشکی جهرم

چکیده

زخم پای دیابتی، که از عوارض دیابت شیرین است، با همراه شدن عفونت در زخم می تواند خطر قطع عضو و یا حتی مرگ را افزایش دهد. باکتری E coli ، که حضور آن در زخم پای دیابتی گزارش شده است، می تواند به وخامت وضعیت زخم منجر شود. مطالعه حاضر با هدف شناسایی ژن های بتالاکتاماز در اشرشیا کلی جداشده از زخم پای دیابتی به روش Multiplex PCR در شهرستان جهرم انجام گرفت.
روش کار:
تعیین حساسیت آنتی بیوتیکی اشرشیا کلی جدا شده با استفاده از روش دیسک دیفیوژن صورت گرفت. شناسایی سویه‏های تولید کننده آنزیم‏های ESBL به کمک روش‏ Double disk synergy test انجام گرفت. به منظور شناسایی ارگانیسم‏های مقاوم به کارباپنم در محیط TSB غلظت 0.5 مک فارلند  استفاده شد. برای جداسازی و شناسایی سویه های تولید کننده MBL  از نوارهای  (AB BioDisk, Solna, Sweden) Etest MBL بر طبق دستورالعمل شرکت سازنده استفاده شد. در آزمایش multiplex PCR با استفاده از پرایمرهای اختصاصی وجود ژن های کدکننده آنزیم های بتالاکتاماز شامل blaTEM، blaSHV و blaOXA-1 مورد مطالعه قرار گرفت.

کلیدواژه‌ها

عنوان مقاله [English]

Identification of β-lactamase genes in Escherichia coli isolated from diabetic foot ulcer by Multiplex PCR in Jahrom city

نویسندگان [English]

  • mojtaba akrami
  • zahra karga jahromi

چکیده [English]

Diabetic foot ulcers, which are complications of diabetes mellitus, can be accompanied by an infection in the wound, which can increase the risk of amputation or even death. E coli bacteria, which have been reported to be present in diabetic foot ulcers, can lead to worsening of the condition of the wound. The aim of this study was to detect β-lactamase genes in Escherichia coli isolated from diabetic foot ulcer by Multiplex PCR in Jahrom city.
method:
Determination of antibacterial sensitivity of Escherichia coli isolated using disc diffusion method. The detection of strains producing ESBL enzymes was performed using Double disk synergy test. In order to detect carbapenem resistant organisms in the TSF, the concentration of 0.5 McFarland was used. To isolate and identify MBL-producing strains, the Etest MBL (AB BioDisk, Solna, Sweden) tapes was used according to the manufacturer's instructions. In a multiplex PCR assay, using specific primers, the presence of genes encoding beta-lactamase enzymes including blaTEM, blaSHV and blaOXA-1 was studied.

کلیدواژه‌ها [English]

  • Diabetic Foot Ulcer
  • E coli
  • Antibiotic resistance
  • beta-Lactamase
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