مجله علوم پزشکی پارس

بررسی تولید زیستی و اثر ضدباکتریایی نانوذرات نقره تولید شده توسط عصاره گیاه دارویی چویل ( .Ferulago angulata Boiss)

نویسندگان

1 دانشگاه صنعتی خاتم الانبیاء بهبهان

2 دانشگاه شهید اشرفی اصفهانی

چکیده

مقدمه: نانوذرات نقره در علم پزشکی کاربردهای بسیاری دارد که اثرات وابسته به انداره ذره‏ای دارند. سنتز نانوذرات نقره به دلیل کاربردهای فراوان در شناسایی عوامل بیماری‏زا، درمان سرطان و دارورسانی هدفمند، بسیار ارزشمند است. هدف از این پژوهش تولید نانوذرات نقره با استفاده از عصاره گیاه Ferulago angulata و بررسی فعالیت ضدمیکروبی نانوذرات می‌باشد.
روش کار: بدین منظور عصاره بدست آمده از گیاه‌ چویل به مدت 24، 48، 72 و 96 ساعت در معرض محلول نیترات نقره 1/. مولار با غلظت‌های مختلف (2/5,5,7/5,10,15 و15میلی مول)قرار گرفت. بعد از تغییر رنگ به سمت قهوه‏ای، با استفاده از آنالیزهای UV-visible ، DLS، SEM، TEM، XRD و FTIR وجود نانو ذرات نقره اثبات شد.
یافته ها: طیف سنجی UV-visible نشان داد که  نانوذرات نقره دارای پیک جذب در 450 نانومتر بودند. با توجه به نتایج آنالیزهای صورت گرفته قطر متوسط نانوذرات 19 نانومتر با حدود اندازه ذرات 3 تا  62 نانومتر و به شکل کروی بودند. در نهایت فعالیت ضدمیکروبی نانوذرات نقره علیه باکتری‌های استافیلوکوکوس‌اورئوس، باسیلوس‌سرئوس، اشریشیا‌کُلی و سودوموناس‌آئروژینوزا بررسی و اثبات شد.
نتیجه گیری: نتایج حاصل کارکرد خوب این گیاه را جهت سنتز نانوذرات به وضوح نشان می‌دهد، بنابراین مـی‏توانـد در درمان بسیاری از بیماری‏های منتقل شده توسط باکتری در زمینه پزشکی مفید واقع شود.
 

کلیدواژه‌ها

عنوان مقاله [English]

Investigating the bioavailability and antibacterial effect of silver nanoparticles produced by ( ferulago angulate Boiss.) extract

نویسندگان [English]

  • Zahra Zarei 1
  • Damoun Razmjoue 1
  • Javad Karimi 2

1

2

چکیده [English]

Introduction:
Silver nanoparticles have a wide range of aplications in medical sciences which their efects depend on their size. Synthesis of Silver nanoparticles is very valuable due to its numerous applications in detection of pathogens, cancer therapy and targeted drug delivery.  The purpose of this research is to production of silver nanoparticles by using the aerial parts extract of Ferulago angulata extract and Check Antimicrobial activity of nanoparticles.

Material and Method:


To achieve this purpose, the obtained extract from Ferulago angulata Plant, was exposed to the silver nitrate solution of 0.1 molar with different concentrations for duration of 24,48, 72 and 96 hours. After changing the color to brown, by using UV-visible, DLS, SEM, TEM, XRD and FTIR analyses the existence of Nano silver particle was proved. 
Results:
The UV-visible spectroscopy showed that silver nanoparticles had an absorption peak at 450 nm. By considering the results of conducted analyses, the mean diameter of particles was 19 nm and the size of particles about 3 to 62 nm and were spherical shape. Finally, the antimicrobial activity of silver nanoparticles against the Staphylococcus aureus, Bacillus cereus, Escherichia coli and Pseudomonas aeruginosa was investigated and proved.
Conclusion:
The obtained results show the good functioning of this plant for the synthesis of nanoparticles clearly. So, It can be useful in the treatment of many diseases transmitted by the bacteria in the medical field.
 

کلیدواژه‌ها [English]

  • Biosynthesis
  • Silver nanoparticles
  • Antibacterial
  • Ferulago angulata
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مجله علوم پزشکی پارس
دوره 16، شماره 1 - شماره پیاپی 43
فروردین 1397
صفحه 24-34