مجله علوم پزشکی پارس

اثر کاتچین بر سطح سرمی سایتوکین‌های التهابی، فعالیت آنزیم های آنتی اکسیدانی و آسیب اکسیداتیو DNA بافت تخمدان در موش های صحرایی مدل سندرم تخمدان پلی کیستیک

نویسندگان

1 دانشگاه پیام نور

2 دانشگاه آزاد اسلامی مشهد

چکیده

مقدمه: اختلالات هورمونی به­همراه استرس­اکسیداتیو و التهاب در بافت تخمدان موجب عدم تخمک­گذاری در مبتلایان به سندرم تخمدان پلی­کیستیک (PCOS) می­شود. کاتچین از مهم­ترین فلاونوئیدهای چای سبز (Camellia sinensis) می­باشد. با توجه به خواص آنتی­اکسیدانی کاتچین، هدف از این مطالعه تعیین اثر کاتچین بر سطح سرمی سایتوکین­های التهابی، فعالیت آنزیم­های آنتی­اکسیدانی و آسیب اکسیداتیو DNA بافت تخمدان در موش­های صحرایی مدل سندرم تخمدان پلی­کیستیک می­باشد.

روش­ کار: در این مطالعه تجربی، تعداد 24 سر موش صحرایی ماده نژاد ویستار به 4 گروه مساوی (6=n) شاهد (محلول سالین، 24 روز، تزریق داخل صفاقی)، PCOS تیمار نشده (محلول سالین، 24 روز، تزریق داخل صفاقی) و  PCOSتحت تیمار با کاتچین (50 و 100 میلی­گرم بر کیلوگرم، 24 روز، تزریق داخل صفاقی) تقسیم شدند. سندرم تخمدان پلی­کیستیک در گروه­های مبتلا به PCOS، با یک­بار تزریق عضلانی استرادیول والرات (40 میلی­گرم بر کیلوگرم) القاء شد. در پایان دوره درمان، سطح سرمی TNF-α، IL-1β و IL-6، همچنین سطح آنزیم­های SOD،CAT ، GPX و میزان MDA و HOdG-8 در بافت تخمدان توسط روش الایزا سنجش شد.

یافته­ها: در مقایسه با گروه PCOS تیمار نشده، سطح سرمی TNF-α، IL-1β و IL-6 در گروه­های تیمار شده با غلظت­های 50 و 100 میلی­گرم بر کیلوگرم کاتچین، وابسته به دوز تزریقی به­طور معنی­داری کاهش، سطح آنزیم­های SOD،CAT ، GPX بافت تخمدان به­طور معنی­داری افزایش و میزان MDA و HOdG-8 به­طور معنی داری کاهش یافت (05/0>p < /span>).

نتیجه­گیری: کاتچین موجب کاهش سطح سرمی سایتوکاین­ها، افزایش فعالیت آنزیم­های آنتی­اکسیدانی و کاهش پراکسیداسیون لیپیدی و آسیب اکسیداتیو DNA بافت تخمدان در موش­های صحرایی مدل سندرم تخمدان پلی­کیستیک می­شود.

کلیدواژه‌ها

عنوان مقاله [English]

Effect of Catechin on Serum Levels of Inflammatory Cytokines, Antioxidant Enzymes Activity and DNA Oxidative Damage of Ovarian Tissue in Polycystic Ovarian Syndrome Rat Model

نویسندگان [English]

  • Rahbareyan Raheleh 1
  • sadoghi damoon 2

1

2

چکیده [English]

Introduction:

Hormonal disorders associated with oxidative stress and inflammation in ovarian tissue causing anovulation in patients with polycystic ovary syndrome (PCOS). Catechin is the most important flavonoids from green (Camellia sinensis). According to the antioxidant properties of catechin, the aim of this study was to determine the effects of catechin on serum levels of inflammatory cytokines, antioxidant enzymes activity and DNA oxidative damage of ovarian tissue in polycystic ovarian syndrome rat model.

Materials & methods:

In this experimental study, 24 Wistar male rats were divided into 4 equal groups (n=6) of control (saline solution, 24 days, ip), non-treated PCOS (saline solution, 24 days, ip) and PCOS treated with Catechin (50 and 100 mg/kg, 24 days, ip). Polycystic ovarian syndrome was induced by single intramuscular injection of estradiol valerate (40 mg/kg) in PCOS groups. At the end of treatment period, serum level of TNF-α, IL-1β and IL-6, also level of SOD, CAT, GPX enzymes and level of MDA and HOdG-8 in ovarian tissue were measured by ELISA.

Results:

Compared to non-treated PCOS group, serum level of TNF-α, IL-1β and IL-6 in groups of treated with 50 and 100 mg/kg of catechin, dose-dependent significantly decreased, level of SOD, CAT, GPX enzymes in ovarian tissue significantly increased and level of MDA and HOdG-8 was significantly decreased (p<0.05).

Conclusion:

Catechin is decreases serum level of cytokines, increase activity of antioxidant enzymes and decreases lipid peroxidation and DNA oxidative damage of ovarian tissue in polycystic ovarian syndrome rat model.

کلیدواژه‌ها [English]

  • Polycystic ovarian syndrome
  • Catechin
  • Cytokine
  • Oxidative stress
  • Rat
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مجله علوم پزشکی پارس
دوره 15، شماره 1 - شماره پیاپی 39
فروردین 1396
صفحه 23-35