مجله علوم پزشکی پارس

بررسی اثر پیش تغذیه عصاره زیره سبز (Cuminum cyminum) بر افزایش نفوذپذیری سد خونی- مغزی حاصل از سکته مغزی در موش صحرایی

نویسندگان

1 دانشگاه آزاد اسلامی زنجان

2 مرکز تحقیقات بیولوژیک دانشگاه آزاد اسلامی زنجان

چکیده

مقدمه: سکته مغزی مهمترین بیماری نورولوژیک و سومین عامل مرگ و میر بعد از بیماری های قلبی و سرطان در افراد مسن می باشد. با وجود تلاش های زیاد در جهت درمان یا فهم پاتوفیزیولوژی سکته مغزی هنوز درمان موثری برای آن یافت نشده است. گیاه زیره سبز با نام علمی Cuminum cyminum در درمان بیماری ها به کار می رود. این مطالعه با هدف بررسی اثر عصاره زیره سبز بر کاهش آسیب سد خونی- مغزی حاصل از سکته مغزی انجام گرفت. روش کار: در این مطالعه تجربی از 5 گروه 6 تایی موش های صحرایی نر استفاده شد. گروه ها شامل شم، کنترل و 3 گروه آزمایشی بودند که دوز های 25 ،50 و100میلی گرم بر کیلو گرم وزن بدن عصاره زیره سبز را به صورت خوراکی به مدت 30 روز دریافت نمودند. 2 ساعت بعد از آخرین گاواژ گروه های کنترل و آزمایشی تحت جراحی انسداد شریان میانی مغز به منظور القای ایسکمی و اندازه گیری استحکام سد خونی- مغزی قرار گرفتند. نتایج: غلظت اوانس آبی در نیمکره آسیب دیده (نیمکره راست) مغز در گروه کنترل در مقایسه با دوزهای 25 و 50 عصاره تفاوت معنی داری نداشت. اما دوز 100 نسبت به گروه کنترل تفاوت معنی داری را نشان داد به طوری که میزان اوانس آبی در نیمکره راست کاهش یافت (05/0>p). نتیجه گیری: پیش تیمار با عصاره خوراکی زیره سبز با دوز 100 (میلی گرم بر کیلو گرم وزن بدن) باعث کاهش آسیب سد خونی- مغزی حاصل از سکته مغزی می گردد.

کلیدواژه‌ها

عنوان مقاله [English]

Evaluation of the effect of Cuminum cyminum extract pre-treatment on increased permeability of blood-brain barrier caused by stroke in rat

نویسندگان [English]

  • mahdis mansouri 1
  • mehdi rahnema 1
  • maesumeh eslami 2

1

2

چکیده [English]

Introduction: stroke is most important neurological disease and the third cause of mortality after heart diseases and cancer in the elderly. Despite many efforts for the treatment or understanding the pathophysiology of stroke, an effective treatment has not been found yet. The Cumin plant with the scientific name "Cuminum cyminum" is used for the treatment of diseases. This study was conducted the effect of cumin on reduce the damage of blood-brain barrier resulting from stroke. Methods & Materials: In this experimental study, the male rats were divided in 5 groups of 6. Groups including sham, control and 3 experimental groups that were received 25, 50, 100 mg/kg of cumin extract orally for 30 days. 2h after the last gavage, control and experimental groups were underwent middle cerebral artery occlusion surgery to induce stroke and measure the stability of blood-brain barrier. Results: Concentration of Blue Evans in the damaged hemisphere (right hemisphere) in the control group had no significant difference compared with the doses of 25 and 50 of cumin extract. But dose of 100 showed a significant difference compared to the control group as Blue Evans in the damaged hemisphere decreased (P <0.05). Conclusion: Pre-treatment with 100 mg/kg of cumin extract reduces damage of the blood-brain barrier resulting from stroke.

کلیدواژه‌ها [English]

  • Stroke
  • cumin
  • blood-brain barrier
  • Rat
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مجله علوم پزشکی پارس
دوره 13، شماره 4 - شماره پیاپی 34
دی 1394
صفحه 1-6