نقش محافظتی عصاره چای سبز بر عملکرد بافت تخمدان روی موش های صحرایی تیمارشده با حشره کش مالاتیون

نویسندگان

دانشگاه آزاد اسلامی واحد جهرم

چکیده

مقدمه و هدف: این مطالعه به منظور بررسی نقش محافظتی عصاره چای سبز بر عملکرد بافت تخمدان روی موش های صحرایی تیمار شده با حشره کش مالاتیون انجام گرفت. روش کار: در این پژوهش، از 72 سر موش صحرایی بالغ ویستار، 3-2 ماهه(باوزن تقریبی15±200گرم)استفاده گردیده است. حیوانات به 9گروه 8 تایی تقسیم شدند. کنترل دارویی دریافت نکرد. شاهد‌ 2/0 سی‌سی سرم فیزیولوژی و تجربی1، 2و3 به ترتیب مقادیرmg/Kg B.W.400 و 200،100 عصاره آبی چای و تجربی4 ‌ mg/Kg B.W40 مالاتیون و تجربی5،6و7 به ترتیب مقادیر mg/Kg B.W. 400 و 200،100 عصاره چای و mg/Kg B.W.40 مالاتیون دریافت کردند. پس از گذشت 15 روز از قلب موش‌ها خونگیری شد و از سرم تهیه شده، غلظت هورمون‌های جنسی اندازه گیری شد. تخمدانها جهت بررسی تعداد فولیکول‌های تخمدانی جدا گردید. نتایج: غلظت هورمون‌های پروژسترون، استروژن، FSH و LH در گروه تجربی4 کاهش معنی داری در مقایسه با کنترل و سایر گروه‌ها نشان دادند. تعداد فولیکول‌های بدوی و گراف در گروه تجربی 4 کاهش و در گروه تجربی 2 و 3 افزایش معنی دار نسبت به کنترل داشته است. فولیکول‌های آترزی در گروه تجربی4 افزایش معنی‌داری نسبت به سایر گروه‌ها داشت. تعداد فولیکول‌های اولیه، ثانویه و جسم زرد در گروه‌تجربی4 کاهش معنی‌داری در مقایسه با کنترل داشتند. بحث و نتیجه گیری: مالاتیون دارای اثرات سوء بر میزان ترشح هورمونهای جنسی موش‌های ماده صحرایی و فرآیند اووژنز داشته و عصاره چای سبز باعث کاهش اثرات منفی مالاتیون میشود.

کلیدواژه‌ها

عنوان مقاله [English]

Protective effect of green tea extract on ovary tissue function in rats treated by Malathion insecticide

نویسندگان [English]

  • Marzieh alsadat Hoseini shahr khafri
  • Vahid Hemayatkhah jahromi
  • Elaheh Samani Jahromi

چکیده [English]

Abstract: Background: This study was conducted to investigate the protective effect of green tea extract on ovary tissue performance in rats treated by Malathion insecticide. Methods: In this research, 72 Wistar mature rats with ages of 2 to 3 months (approximate weight of 200±15 g) were experimented. Animals were divided into 9-octet groups of animals. Control group did not receive any medication. Control group was given 0.2cc physiological serum and experimental 1, 2 and 3 received 100, 200 and 400 mg/KG B.W. green tea extract respectively, the experimental 4 was given 40 mg/KG B.W. Malathion and the experimental 5,6 and 7 each received 100, 200 and 400 mg/KG B.W. tea extract respectively and 40 mg/KG B.W. Malathion. After 15 days, the rats’ hearts were blooded, and the sex hormones concentration was measured by the prepared serum. Ovaries were then separated to count the ovarian follicles.. Results: Concentration of Estrogen, Progesterone, FSH and LH were significantly decreased in experimental group 4,compared to control and other groups. The primary and Graf follicles in experimental group 4 decreased and in experimental groups 2 and 3 significantly increased in comparison to control group. Atresia folliclesa had a significant increase in experimental group 4,compared to other groups. Primary and secondary follicles’ and corpus luteum in experimental group 4 significantly decreased compared to control group. Conclusions: Malathion had an adverse effect on secretion of sex hormones in female rats, as well as the process of oogenesis, and green tea extract decreased those negative effects.

کلیدواژه‌ها [English]

  • Malathion
  • Green tea
  • Oogenesis
  • Sex Hormones
  • Rat
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