بررسی اثر عصاره هیدروالکلی گیاه بشقابی کوهستانی (Scutellaria multicaulis) در تشنج القا شده با پنتیلن‎ تترازول در موش سوری نر

نوع مقاله : مقاله پژوهشی

نویسندگان
1 مرکزتحقیقات گیاهان دارویی، پژوهشکده علوم پایه سلامت، دانشگاه علوم پزشکی شهرکرد، شهرکرد، ایران
2 مرکز تحقیقات فیزیولوژی کاربردی، دانشگاه علوم پزشکی اصفهان، اصفهان، ایران
چکیده
زمینه و هدف: تشنج یکی از بیماری های صعب العلاج امروز جامعه بشری است که محققین بدنبال راههای درمانی بهتر و داروهای بیشتر در این زمینه هستند. این مطالعه به منظور کمک به یافتن داروی گیاهی موثر و بدون عوارض جانبی در این خصوص طراحی و اجرا شد.
روش بررسی: گیاه بشقابی کوهستانی به روش ماسراسیون عصاره‎گیری شد. 48 سر موش سوری نر به‌صورت تصادفی به 6 گروه 8 تایی تقسیم شدند: 1- گروه کنترل، 2- گروه سالین و پنتیلن تترازول (مدل تشنج)، گروه‌های 3 تا 5 که عصاره هیدروالکلی گیاه بشقابی کوهستانی را به مدت یک هفته در دوزهای 30، 90 و 150 میلی گرم بر کیلوگرم بصورت داخل صفاقی دریافت کردند. و در روز هفتم با تزریق وریدی پنتیلن تترازول در آنها تشنج القاء شد. گروه 6 یا کنترل مثبت که به جای عصاره به مدت یک هفته داروی فنوباربیتال با دوز 4 میلی گرم بر کیلوگرم بصورت داخل صفاقی دریافت کردند. مدت زمان تاخیر در شروع تشنج و ظرفیت کل آنتی‌اکسیدانی و مالون‌دی‌آلدهید هیپوکمپ و سرم در آنها اندازه گیری شد..
یافته‌ها: هر سه دوز عصاره سبب افزایش مدت زمان تاخیر در شروع تشنج شد. عصاره گیاه بشقابی کوهستانی سبب افزایش ظرفیت کل آنتی اکسیدانی هیپوکمپ و سرم شد. میزان مالون دیی آلدئید در گروههای دریافت کننده عصاره کاهش معنی داری نشان داد(0.001p<).
نتیجه‌گیری: عصاره هیدروالکلی گیاه بشقابی کوهستانی ضمن نشان دادن اثرات ضد تشنجی، سبب کاهش استرس اکسیداتیو از طریق افزایش ظرفیت کل آنتی اکسیدانی و کاهش میزان مالون دی آلدئید هیپوکمپ و سرم شد.
کلیدواژه‌ها

عنوان مقاله English

Investigation of the Effects of Hydroalcoholic Extract of Scutellaria Multicaulis on Pentylenetetrazole-Induced Seizures in Male Mice

نویسندگان English

najmeh asgharzadeh 1
maedeh mazaheri 1
zahra lorigoeini 1
hossein Amini-Khoei 1
Marzieh Mardani 1
Elham Bijad 1
Diana shahrani 1
Mohamad shahrani 2
mehrdad shahrani korrani 1
1 Medical Plants Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran
2 Applied Physiology Research Center, Isfahan University of Medical Sciences, Isfahan, Iran
چکیده English

Background and Objective: Seizures are one of the most incurable diseases in human society today, and researchers are looking for better treatment methods and more drugs in this field. This study was designed and implemented to help find an effective herbal medicine without side effects in this regard.
Methods: Scutellaria multicaulis was extracted by maceration method. Forty eight male mice were randomly divided into 6 groups of 8: 1- control group, 2- saline and pentylenetetrazole group (seizure model), groups 3 to 5 received hydroalcoholic extract of Scutellaria multicaulis intraperitoneally for one week at doses of 30, 90 and 150 mg/kg. And on the seventh day, seizures were induced by intravenous injection of pentylenetetrazole. Group 6 or positive control, which received phenobarbital at a dose of 4 mg/kg intraperitoneally for one week instead of the extract, The latency to seizure onset and the total antioxidant capacity and malondialdehyde of the hippocampus and serum were measured in them.
Results: All three doses of the extract increased the latency to seizure onset. The extract of the Scutellaria multicaulis increased the total antioxidant capacity of the hippocampus and serum. The level of malondialdehyde in the groups receiving the extract showed a significant decrease (p<0.001).
Conclusion: The hydroalcoholic extract of the Scutellaria multicaulis, while showing anticonvulsant effects, reduced oxidative stress by increasing the total antioxidant capacity and reducing the level of malondialdehyde of the hippocampus and serum.

کلیدواژه‌ها English

Seizures
Pentylenetetrazole
Malondialdehyde
Total Antioxidant Capacity
Hippocampus
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