تأثیر تمرین مقاومتی بر پروتئین‌های مرتبط با رتیکولوفاژی (ATF6، mTOR و JNK1) بطن چپ قلب موش‌های صحرایی نر بالغ مبتلا به دیابت نوع 2

نوع مقاله : مقاله پژوهشی

نویسندگان
1 گروه علوم ورزشی، مؤسسه‌ی آموزش عالی آپادانا، شیراز، ایران.
2 گروه آموزش تربیت بدنی، دانشگاه فرهنگیان، تهران، ایران.
چکیده
مقدمه: با افزایش شیوع دیابت در سراسر جهان، به نظر می‌رسد تمرین مقاومتی می‌تواند اختلال عملکرد قلب را در مدل‌های دیابتی کاهش دهد. هدف از مطالعه حاضر اثربخشی تمرین مقاومتی بر پروتئین‌های مرتبط با رتیکولوفاژی در بطن چپ قلب رت‌های دیابتی نوع 2 است.
روش‌کار: در این مطالعه‌ تجربی، 12 سر رت نر 2 ماهه از نژاد اسپراگ‌‌داولی با میانگین وزن 20±280 گرم انتخاب شدند. دیابت نوع 2 از طریق تزریق محلول‌های نیکوتین‌آمید و استرپتوزوتوسین القاء شد. رت‌ها به روش تصادفی به گروه تمرین مقاومتی دیابتی و کنترل دیابتی تقسیم شدند. تمرین مقاومتی شامل 8 هفته، هفته‌ای 3 جلسه بالارفتن از نردبان عمودی با شیب 85 درجه به طول یک متر با 26 پله و 2 سانتی‌متر فضای بین هر پله بود. برای تجزیه و تحلیل داده ها از آزمون های t-مستقل و t-وابسته در نرم افزار SPSS نسخه 29 استفاده شد.
یافته‌ها: محتوای پروتئین ATF6 (P= 0.001) و mTOR (P=0.03) به ترتیب کاهش و افزایش معنی‌داری را نسبت به گروه کنترل در بطن چپ قلب پس از 8 هفته تمرین مقاومتی نشان دادند. از طرفی تغییر معنی‌داری در محتوای پروتئین JNK1 در بطن چپ قلب مشاهده نشد (P=0.16). میزان قند خون (mg/dl) بین هفته هشتم نسبت به هفته اول در گروه تمرین مقاومتی تغییر معنی داری را نشان نداد (0.07=P)، اما بین هفته هشتم در گروه کنترل نسبت به گروه تمرین مقاومتی تفاوت معنیداری مشاهده شد (0.001=P).
نتیجه‌گیری: تمرین مقاومتی به مدت 8 هفته می تواند به تعدیل پروتئین های مربوط به رتیکولوفاژی در قلب افراد مبتلا به دیابت نوع 2 کمک کند.
کلیدواژه‌ها

عنوان مقاله English

The effect of resistance training on reticulophagy-related proteins (ATF6, mTOR and JNK1) in the left ventricle of adult male rats with type 2 diabetes

نویسندگان English

Fatemeh Solhdoust 1
Hamed Alizadeh Pahlavani 2
Mohammad Sherafati Moghadam 1
1 Department of Sport Sciences, Apadana Institute of Higher Education, Shiraz, Iran.
2 Department of Physical Education, Farhangian University,, Tehran, Iran
چکیده English

Introduction: With the increasing prevalence of diabetes worldwide, it seems that resistance training can reduce cardiac dysfunction in diabetic models. The aim of the present study is the effectiveness of resistance training on reticulophagy-related proteins in the left ventricle of the heart of type 2 diabetic rats.
Materials and Methods: In this experimental study, 12 2-month-old male Sprague Dawley rats with an average weight of 280±20 grams were selected. Type 2 diabetes was induced by injecting nicotinamide and streptozotocin solutions. Rats were randomly divided into the diabetic resistance training group and the diabetic control group. Resistance training consisted of 8 weeks and 3 sessions per week of climbing a vertical ladder with an 85degree slope, one meter long with 26 steps and 2 cm space between each step. To analyses the data, independent t-tests, and t-dependent tests were used in SPSS version 29 software.
Results: The protein content of ATF6 (P=0.001) and mTOR (P=0.03) showed a significant decrease and increase, respectively, compared to the control group in the left ventricle of the heart after 8 weeks of resistance training. On the other hand, there was no significant change in JNK1 protein content in the left ventricle of the heart (P=0.16). The level of blood sugar (mg/dl) did not show a significant change between the eighth week compared to the first week in the resistance training group (P=0.07), but a significant difference was observed between the eighth week in the control group compared to the resistance training group (P=0.001).
Conclusion: It seems that resistance training for 8 weeks is capable of modulating proteins related to reticulophagy in the heart of people with type 2 diabetes.

کلیدواژه‌ها English

Left Ventricle
Resistance Exercise
Reticulophagy
Type 2 Diabetes
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