مجله علوم پزشکی پارس
اثر تمرین مقاومتی بر سطح آنزیمهای آنتیاکسیدانی بافت کبدی در موش صحرایی نر ویستار مبتلا به کبد چرب غیرالکلی
نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه علوم ورزشی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد ایلام، ایلام، ایران
2 گروه فیزیولوژی ورزشی، دانشکده علوم انسانی، دانشگاه آزاد اسلامی واحد ایلام، ایلام، ایران
چکیده
مقدمه: مکانیسم زیربنایی بیماری کبد چرب غیرالکلی (NAFLD) و پیشرفت آن به استئوهپاتیت غیرالکلی (NASH) به خوبی شناخته نشده است. هدف از اجرای این پژوهش، تعیین اثر تمرین مقاومتی بر سطح آنزیمهای آنتیاکسیدانی در بافت کبدی موشهای صحرایی نر نژاد ویستار مبتلا به کبد چرب غیرالکلی بود.
روش کار: در این مطالعه تجربی، 21 سر موش صحرایی نر بالغ نژاد ویستار به طور تصادفی به سه گروه تقسیم شدند: کنترل، رژیم پرچرب و رژیم پرچرب+ تمرین مقاومتی. حیوانات گروههای رژیم پرچرب به مدت 23 هفته رژیم پرچرب شامل ترکیبی از فروکتوز، کربن تتراکلرید و روغن زیتون دریافت کردند. گروه تمرینی ضمن دریافت رژیم پرچرب، 8 هفته پایانی پروتکل تمرین مقاومتی فزاینده شامل حمل وزنه معادل 30 تا 100 درصد وزن بدن را سه جلسه در هفته اجرا کردند. در پایان مداخله، سطح آنزیمهای سوپراکسید دیسموتاز (SOD) و گلوتاتیون پراکسیداز (GPX) در بافت کبد به روش رنگ سنجی آنزیمی اندازه گیری شد. همچنین، آسیب شناسی کبد برای تأیید نتایج بیوشیمیایی انجام گرفت. آزمونهای تحلیل واریانس یک طرفه و تعقیبی توکی جهت تجزیه و تحلیل داده های جمع آوری شده استفاده شد.
یافته ها: سطح آنزیم SOD کبدی گروه رژیم پرچرب+ تمرین مقاومتی در مقایسه با گروه رژیم پرچرب به طور معناداری افزایش یافت (0.03 = p)، اما سطح آنزیم GPX کبدی در هر سه گروه تغییر معناداری نداشت (0.08 = p). بررسی آسیب شناسی کبد نیز تغییرات ناشی از رژیم پرچرب و آثار محافظتی تمرین مقاومتی را تأیید نمود.
نتیجه گیری: طبق نتایج این مطالعه می توان گفت اجرای تمرین مقاومتی می‌تواند اختلال آنزیمهای آنتی اکسیدانی و استئاتوز بافت کبدی ناشی از دریافت رژیم غذایی پرچرب در موشهای صحرایی نژاد ویستار مبتلا به کبد چرب غیر الکلی را به طور معناداری بهبود بخشد.
کلیدواژه‌ها
عنوان مقاله [English]
The effect of resistance training on the level of liver antioxidant enzymes of male Wistar rats with non-alcoholic fatty liver
نویسندگان [English]
- Sara Kianitabar 1
- Abdolhossein Taheri Kalani 2
- Mahmoud Nikseresht 1
1 Department of Sport Sciences, Faculty of Humanities, Ilam Branch, Islamic Azad University, Ilam, Iran
2 Dep of Exercise Physiology, Faculty of Humanities, Ilam Branch, Islamic Azad University, Ilam, Iran.
چکیده [English]
Introduction: The pathogenic mechanism underlying non-alcoholic fatty liver disease (NAFLD) and progression from non-alcoholic fatty liver (NAFL) to non-alcoholic steatohepatitis (NASH) is not entirely understood. The purpose of this study was to determine the effect of resistance training on the level of antioxidant enzymes in liver tissue of male Wistar rats with non-alcoholic fatty liver.
Materials and Methods: In the present experimental study, 21 adults male Wistar rats were purchased and randomly divided into three groups: control, high fat diet and high fat diet+ resistance training groups. Animals in the high-fat diet groups received a high-fat diet including a combination of fructose, carbon tetrachloride and olive oil for 23 weeks. The training group while receiving a high-fat diet, in the final 8 weeks performed the progressive resistance training protocol including carrying weights equivalent to 30 to 100 percent of body weight three sessions a week. At the end of the intervention, level of superoxide dismutase (SOD) and glutathione peroxidase (GPX) enzymes in liver tissue were measured by enzyme colorimetric method. Also, liver histopathology was performed to confirm the biochemical results. The data collected were analyzed using statistical tests of one-way analysis of variance and Tukey's follow-up test.
Results: Liver SOD enzyme level in the high-fat diet+ resistance training group showed significant increase in compared to the high-fat diet group (p = 0.035), but liver GPX enzyme level did not change significantly in the three groups (p = 0.085). The histopathological examination of the liver also confirmed the changes caused by the high-fat diet and the protective effects of resistance training.
Conclusion: According to the results of this study, it can be said that resistance training can significantly improve the disorder of antioxidant enzymes and liver tissue steatosis caused by high-fat diet in male Wistar rats with non-alcoholic fatty liver.
کلیدواژه‌ها [English]
- Diet
- Resistance training
- Antioxidant
- Oxidative stress
- Fatty liver
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