مجله علوم پزشکی پارس

ساخت فرمولاسیون لیپوزومی حاوی پاکلی تاکسل و مقایسه سمیت آن با پاکلی تاکسل لیپوزومه نشده بر رده‌ی سلولی MCF-7 سرطان پستان

نویسندگان

1 کارشناسی ارشد ژنتیک، گروه زیست شناسی، دانشگاه آزاد اسلامی واحد تهران مرکز، تهران، ایران.

2 استادیار، گروه زیست شناسی، دانشگاه آزاد اسلامی واحد تهران مرکز، تهران، ایران.

3 کارشناسی ارشد زیست شناسی سلولی و مولکولی، گروه زیست فناوری، شرکت ریز زیست فناوران فردا نگر، پارک علم و فناوری یزد، ایران.

4 استادیار، مرکز تحقیقات نانوتکنولوژی پزشکی و مهندسی بافت، پژوهشکده علوم تولید مثل ، دانشگاه علوم پزشکی شهید صدوقی یزد، ایران.

چکیده

مقدمه: شیمی درمانی یکی از روش های رایج در درمان سرطان است که همواره با چالش هایی روبرو بوده است. هدف از پژوهش حاضر ساخت نانوحامل های حاوی داروی شیمی درمانی پاکلی تاکسل و بررسی سمیت آن بر رده سلولی MCF-7 سرطان پستان بود.
روش کار: سه فرمولاسیون از نانو حامل های لیپوزومی حاوی پاکلی تاکسل با غلظت های متفاوتی از فسفاتیدیل کولین و کلسترول با استفاده از روش فیلم لایه نازک ساخته شد. سپس با توجه به میزان بارگذاری دارو، یکی از فرمولاسیون ها انتخاب و پگیله شد. درصد بارگذاری دارو درون نانولیپوزوم پگیله، الگوی رهایش دارو در شرایط سلول سالم و سرطانی، اندازه و بار سطحی نانو ذرات با استفاده از DLS و شکل ظاهری نانو ذرات با استفاده از میکروسکوپ پویش الکترونیکی بررسی شد. در پایان سمیت سامانه لیپوزومی حاوی دارو و داروی لیپوزومه نشده بر رده سلولیMCF-7 با استفاده از روشMTT  مورد بررسی قرار گرفت.
یافته­ ها: درصد بارگذاری دارو، اندازه، پتانسیل زتا نانو حامل های پگیله شده حاوی دارو به ترتیب 2.35±90.6 درصد، nm 49.4 و mV 46.74±5.55- بود. رهایش دارو از سامانه لیپوزومی طی 72 ساعت در شرایط سلول سالم و سرطانی آهسته بود. شکل ظاهری نانو ذرات صاف و کروی بوده و هیچ برهم کنش شیمیایی میان دارو و نانو حامل مشاهده نشد. همچنین داروی پاکلی تاکسل لیپوزومه در مقایسه با داروی لیپوزومه نشده سمیت بیشتری روی رده سلولی MCF-7 سرطان پستان داشت.
نتیجه­­ گیری: با توجه به برخورداری از ویژگی های فیزیکوشیمیایی مناسب، فرمولاسیون لیپوزومی حاصل از مطالعه حاضر را می توان برای انجام پژوهش های بیشتر در زمینه سرطان پستان پیشنهاد کرد.

کلیدواژه‌ها

عنوان مقاله [English]

Fabrication of liposomal formulation containing paclitaxel and comparison of its toxicity with non-liposomal paclitaxel on MCF-7 breast cancer cell line

نویسندگان [English]

  • Elmira Rabani 1
  • Roudabeh Behzadi 2
  • Mohammad Majdizadeh 3
  • Bibi Fatemeh Haghiralsadat 4

1 MSc. Department of Biology, Islamic Azad University Central Tehran Branch, Tehran, Iran

2 Assistant Professor, Department of Biology, Islamic Azad University Central Tehran Branch, Tehran, Iran

3 MSc. Nano-Biotech Foresight Company Biotechnology Campus, Science & Technology Park of Yazd, Yazd, Iran

4 Assistant Professor, Medical nanotechnology & tissue engineering research center, Yazd reproductive sciences institute, Shahid Sadoughi University of medical sciences, Yazd, Iran

چکیده [English]

Introduction: Chemotherapy is one of the most common methods in cancer therapy that has always faced challenges.
The aim of the present study was to develop nano-carriers containing paclitaxel chemotherapy drug and to investigate its toxicity on MCF-7 breast cancer cell line.
Materials and Methods: Three formulations of liposomal nano-carriers containing paclitaxel with different concentrations of phosphatidylcholine and cholesterol were fabricated using the thin film method. Then according to the loading rate of drug, one of the formulations was selected and pegylated. Percentage of drug loading into pegylated nano-liposomes, drug release pattern in healthy and cancerous cell conditions, size and surface charge of nanoparticles (using DLS) and morphology of nanoparticles (using SEM) was evaluated. At the end, the toxicity of liposomal system containing drug and non-liposome drug on MCF-7 cell line was evaluated by MTT assay.
Result: The results showed that drug loading percentage, size, zeta potential of pegylated nano-carriers containing the drug were 90.6±2.35%, 49.4nm and -46.74±5.55mV, respectively. Release of the drug from the liposomal system is slow within 72 hours in normal and cancerous cell conditions. The morphology of the nanoparticles was smooth and spherical, and no chemical interaction was observed between the drug and the nano-carrier. Paclitaxel liposomal also had more toxicity to MCF-7 cell line breast cancer than non-liposomal drug.
Conclusion: Based on the results, the liposomal formulation of this study can be recommended for further research
in breast cancer with respect to its physicochemical properties.

کلیدواژه‌ها [English]

  • Liposome
  • Paclitaxel
  • MCF-7 cells
  • Neoplasm
  • chemotherapy
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مجله علوم پزشکی پارس
دوره 18، شماره 2 - شماره پیاپی 52
تیر 1399
صفحه 26-40