مجله علوم پزشکی پارس

جداسازی آمیب نگلریا و شناسایی نگلریا آسترالینسیس از آب استخرهای شنای استان کرمان

نویسندگان

1 گروه انگل شناسی و قارچ شناسی پزشکی، دانشکده پزشکی،دانشگاه علوم پزشکی جهرم، جهرم، ایران

2 مرکز تحقیقات لیشمانیوز، دانشکده پزشکی، دانشگاه علوم پزشکی کرمان، کرمان، ایران

3 مرکز تحقیقات بیماریهای زئونوز، دانشکده پزشکی، دانشگاه علوم پزشکی جهرم، جهرم، ایران

چکیده

مقدمه: در بین آمیب های آزادزی، گونه های N.fowleri و N.australiensis علت مرگ ناشی از مننگوآنسفالیت آمیبی اولیه به حساب می‌آیند. از آن جا که آب استخرها منبع آمیب‌های آزادزی است، این مطالعه با هدف جداسازی آمیب نگلریا و شناسایی نگلریا آسترالینسیس از آب استخرهای شنای استان کرمان انجام شد.
روش کار: 80 نمونه آب از 20  استخر استان کرمان در تابستان 1397 جمع‌آوری و میزان کلر باقی‌مانده، pH و دمای آن‌ها اندازه‌گیری شد. نمونه‌های آب فیلتر و کشت داده شدند. پس از شناسایی مورفولوژیکی آمیب، تعیین جنس به روش  PCR و تایید گونه با مقایسه توالی محصول PCR در بانک ژن انجام شد.
یافته‌ها: دما و باقیمانده کلر به ترتیب در 50% و 93.7 %  از استخرها به میزان استاندارد بود. به طورکلی 8.75 % از نمونه آب استخرها، آلوده به آمیب با مورفولوژی نگلریا بودند. نتیجه PCR حاکی از وجود نگلریا در هفت نمونه بود. درصد آلودگی در سانس آقایان و در یک متری کناره استخر  بیشتر بود. توالی محصول PCR درصد بالایی از هم پوشانی و شباهت با توالی های ثبت شده N.australiensis نشان داد که در بانک ژنی NCBI با شماره MT292609 به ثبت رسید.
نتیجه‌گیری: نتایج پژوهش حاضر نشان داد که آب 25% از استخرهای شنا در استان کرمان به نگلریا آسترالینسیس آلوده هستند. با توجه به توانایی بیماری‌زایی این گونه آمیب در انسان و حیوان، لازم است تمهیدات لازم برای ضدعفونی هر چه بیشتر آب استخرهای شنا توسط دست‌اندرکاران مسائل بهداشتی برای جلوگیری از آلودگی احتمالی اتخاذ شود.

کلیدواژه‌ها

عنوان مقاله [English]

Detection of Naegleria and Identification of Naegleria australiensis from Swimming pools in Kerman Province

نویسندگان [English]

  • Raheleh Eftekhari-Kenzerki 1
  • Kavous Solhjoo 1
  • Zahra Babaei 2
  • Hassan Rezanezhad 3
  • Ahmad Abolghazi 1

1 Department of Medical Parasitology and Mycology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran

2 Leishmaniasis Research Center, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

3 Zoonoses Research Center, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran

چکیده [English]

Introduction: Among the free living amoeba, Naegleria fowleri and Naegleria australiensis are considered as the cause of death due to primary amoebic meningoencephalitis (PAM). As the water of swimming pools are the sources of free living amoeba, this study conducted to detection of Naegleria and identification of N.australiensis from swimming pools.
Materials and Methods: Eighty water samples were collected from 20 swimming pools of Kerman province in summer 2018 and the residual chlorine, pH and temperature were measured, then filtered and cultured. After morphological identification of amoeba, genus identification and species confirmation were performed by PCR and comparison of PCR product sequence in gene bank, respectively.
Results: The temperature and residual chlorine of water were at standard level in 50% and 93.7%, respectively. Overall 8.75% of water swimming pool samples were contaminated with amoeba with similar morphology to Naegleria. The results of PCR were confirmed the present of Naegleria in 7 samples. The contamination percent was higher in men timetable (10%) and in one meter of wall of swimming pools (12.5%). Comparison of PCR products showed the high percentages of Identity and Query Coverage with recorded sequences of N.australiensis and it was deposited in NCBI with accession number MT292609.
Conclusion: The present research showed that 25% of swimming pools in Kerman province are contaminated with
N. australiensis. Regarding to the pathogenic potential of N. australiensis for human and animals, it is necessary, the health workers think about for more disinfection of swimming pools water for preventing of possible infection.

کلیدواژه‌ها [English]

  • Free living amoeba
  • Naegleria australiensis
  • Swimming pool
  • Kerman
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مجله علوم پزشکی پارس
دوره 18، شماره 2 - شماره پیاپی 52
تیر 1399
صفحه 19-25