همراهی پلی مورفیسم ژن اینتر لوکین 19 درموقعیت -513(T/C) باخطرابتلابه عفونت باکتری H.pylori

نویسندگان

1 دانشگاه ازاداسلامی واحد

2 دانشگاه آزاد اسلامی

چکیده

چکیده
مقدمه : هلیکوباکتر پیلوری (H. pylori) یک باسیل تاژکدار، فنری شکل، کم هوازی گرم منفی است که در مخاط معده مستقر شده و باعث بیماری های دستگاه گوارش فوقانی از جمله ورم معده مزمن، زخم معده و سرطان معده می شود.اینتر لوکین 19 به عنوان یک سایتو کاین ضد التهابی در بیماری های گوناگونی ازجمله بیماری های پوستی بیماری های کبدی، بیماری های اتوایمیون و بیماری های میکروبی مورد بررسی و مطالعه قرار گرفته است. ژن این مولکول درموقعیت(rs1028181,-513 T/C)  دارای چند شکلی ژنی می باشد  که در بیان مولکول مذکور تاثیر گذار می باشد.
روش کار: در این مطالعه موردی - شاهدی ، نمونه خونی 100 بیمار و 100 فرد سالم برای بررسی پلی مورفیسم ژن IL-19 در موقعیت   rs1028181  -513T/C در بیماران مبتلا به H.pylori انجام گرفته شد. ژنوتیپ های پلی مورفیسم اشاره شده با روش  PCR-RFLP < /span>تعیین گردید. آنالیز اطلاعات آماری از بررسی ژنومی  بیماران  با استفاده از تست مجذور کای ( Chi square) و لجستیک رگراسیون مورد بررسی قرار گرفت .
یافته ها : بررسی رابطه بین آلل های T و C درموقعیت (rs1028181  -513T/C) نشان دادکه بین آلل C و ابتلا به H.pylori رابطه معناداری وجود دارد ((96/0-45/0  (OR=0.66   CI(( 04/0 =(P < /span>.
نتیجه گیری :با توجه به نتایج به دست آمده به نظر می رسد که آلل C در موقعیت rs1028181  -513T/C)) با خطر ابتلا به باکتری هلیکوباکترپیلوری ارتباط مستقیمی وجود داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

Association of IL-19 gene polymorphism at position -513T/C with risk of H. Pylori

نویسندگان [English]

  • MohammadReza Hadipourfard 1
  • Sirous Naeimi 2

1

2

چکیده [English]

Abstract
Introduction: Helicobacter Pylori (H.pylori) is a flagellated, spiral- shaped, microaerophilic gram negative bacterium that colonizes stomach mucosa and causes upper gastrointestinal diseases, such as gastric inflammation, chronic gastric ulcers and gastric Carcinoma. Chronic inflammation plays an important role in the pathogenesis of autoimmune diseases, allergies and cancer. Inflammatory cytokines, such as IL-19 play an important role in the pathogenesis of diseases caused by H. pylori. Since Helicobacter infection is very important and effects many subjects for a long period, and since it has the potential of converting into cancer, we attempted to examine the role of IL-19 cytokine in induction and continuation of this disease.
Materialand methods:  In this case-control study, blood samples of 100 patient's and 100 healthy individuals was examined for gene polymorphisms of IL-19 at rs1028181-513T/C position in patients with H. pylori infection. Genotype of this polymorphism was determined by RFLP PCR method. Statistical data analysis of genomic and serum studies of patients was carried out by the X 2 (Chi square) and logestic regression.
Results: Investigation of relationship between T and C alleles at rs1028181-513T/C position indicated that there is a significant correlation between allele C and H. pylori infection (p = 0.04 OR = 0/66 (CI; 0/45-0.96)).
Conclusion: It seems that there is a direct association between Helicobacter Pylori infection, and C allele at rs1028181-513T/C position.
 

کلیدواژه‌ها [English]

  • Helicobacter pylori
  • Polymorphism
  • IL-19
  • rs1028181 
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