تأثیر تمرین شنا بر بیان ژن‌های CYP17A1 و CYP19A1 در بافت تخمدان رت‌های ویستار سالم

نویسندگان

دانشگاه شیراز

چکیده

زمینه و هدف: آنزیم‌های CYP17A1 و CYP19A1 پروتئین‌های کلیدی در ساخت و تنظیم هورمون‌های استروئیدی مانند کورتیزول، تستوسترون و استروژن هستند. اما هنوز تاثیر فعالیت ورزشی بر این دو پروتئین مهم در بافت تخمدان بررسی نشده است. هدف از مطالعه حاضر، بررسی تأثیر تمرین شنا بر بیان ژن‌های CYP17A1 و CYP19A1 در بافت تخمدان رت‌های ویستار سالم می‌باشد.
روش بررسی: 20 سر موش صحرایی سالم ماده 2 ماهه از نژاد ویستار با میانگین وزن 20±180 گرم انتخاب ‏شدند و به روش تصادفی به 2 گروه، تمرین (10 سر) و کنترل (10 سر) تقسیم ‏شدند؛ گروه‌ تمرینی 5 روز در هفته  به‏ مدت 6 هفته به تمرین شنا پرداختند. برای تجزیه ‏و تحلیل داده‌ها از آزمون آماری U من-ویتنی استفاده‏ شد.
یافته‌ها: افزایش معناداری در بیان ژن پروتئین‌های CYP17A1  و CYP19A1  در گروه‌ تمرین نسبت به کنترل مشاهده شد(050/0 = P < /span>).
نتیجه‌گیری: با توجه به نقش پروتئین‌های CYP17A1 و CYP19A1 در ساخت هورمون‌های استروئیدی، شش هفته تمرین ورزشی شنا احتمالا می‌تواند یک مداخله مهم و غیردارویی برای درمان اختلالات تخمک‌گذاری و ناباروی باشد باشد.
 
 

کلیدواژه‌ها

عنوان مقاله [English]

The effect of swimming exercise on the expression of CYP17A1 and CYP19A1 genes in ovary tissue of healthy Wistar rats

نویسندگان [English]

  • maryamosadat miri
  • aminollah bahaoddini
  • maryam koushkie jahromi
  • farhad daryanosh
  • , firouzeh gholampour

چکیده [English]

Background and aims: CYP17A1 and CYP19A2 enzymes are key proteins in constructing and regulating steroid hormones such as cortisol, testosterone and estrogen. But the effect of exercise on these two proteins in ovary tissue has not been investigated yet. The purpose of present study was investigating the effect of swimming training on CYP17A1 and CYP19A1 gene expression in ovary tissue of healthy Wistar rats.
 
Method: 20 two months healthy Wistar rats, mean weight of 180±20 gr were selected and divided to two groups of exercise (n=10) and control (n=10) randomly. Exercise group performed swimming five days in a week for 6 weeks. Statistical test of Mann-Whitney was used for data analysis.
 
Results: CYP17A1 and CYP19 gene expression increased significantly in exercise compared to control group (P=0.05).
 
Conclusion: regarding the role of CYP17 and CYP19 in steroid hormone synthesis, probably six weeks of swimming can be an important non pharmacological intervention for treatment of ovulation and infertility problems.
 

کلیدواژه‌ها [English]

  • CYP17A1
  • CYP19A1
  • swimming training
  • ovary tissue
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