مجله علوم پزشکی پارس

اثر مهاری عصاره هیدروالکلی پوست گردو بر کاهش اکسیداسیون لیپوپروتئین با چگالی کم در محیط آزمایشگاهی

نویسندگان

1 وزارت بهداشت درمان وآموزش پزشکی

2 دانشگاه آزاد اسلامی

3 باشگاه خبرنگاران جوان

چکیده

چکیده: مقدمه: اکسیداسیون لیپیدها و از جمله لیپوپروتئین با چگالی کم (LDL) نقش مهمی در ایجاد آترواسکلروز دارد. بااستفاده از مواد آنتی-اکسیدان از قبیل ویتامین E و عصاره هیدروالکلی پوست گردو در جیره غذایی می‌توان از اکسیداسیون LDL جلوگیری کرد و بدین ترتیب مانع ایجاد و پیشرفت آترواسکلروز شد. در مطالعه حاضر، اثرات عصاره هیدروالکلی پوست گردو بر اکسیداسیون LDL سرم ناشی از سولفات مس در محیط آزمایشگاهی بررسی می‌شود. روش کار: از افراد سالم بعد از یک شب ناشتایی نمونه خون گرفته شد. سپس LDLسرم جدا شده گروه های کنترل، اکسید شده با مس و عصاره هیدروالکلی پوست گردو با غلظت های 2/0، 2 و 20 میکروگرم بر میلی‌لیتر مطالعه شدند. برای بررسی اکسیداسیون LDL، مقدار دی‌ان‌های کونجوگه، زمان تاخیری ((Lag time و مالون‌دی‌آلدئید (MDA) حاصل اندازه‌گیری و اثرات غلظت‌های مختلف عصاره هیدروالکلی پوست گردو بر مهار اکسیداسیون LDL سرم در حضور مس بررسی شد. یافته‌ها: نتایج نشان می‌دهد عصاره هیدروالکلی پوست گردو باعث کاهش اکسیداسیون LDLسرم می شود، به طوری که زمان تاخیری در غلظت‌های عصاره 2/0، 2 و 20 میکروگرم بر میلی‌لیتر به ترتیب 87، 178 و 202 درصد می‌باشد. به عبارت دیگر، اثر مهاری عصاره هیدروالکلی پوست گردو بر اکسیداسیون LDL با غلظت عصاره متناسب است. بحث و نتیجه‌گیری: نتایج نشان می دهد عصاره هیدروالکلی پوست گردو از اکسیداسیون LDL جلوگیری می‌کند. چنین ترکیبی ممکن است اثرات مشابهی نیز در موجودات زنده داشته باشد.

کلیدواژه‌ها

عنوان مقاله [English]

The inhibitory effects of Walnut (Juglansregia L.) huskhydroalcoholic extract on LDL oxidation in vitro

نویسندگان [English]

  • Hassan Ahmadvand 1
  • ali khosrobaigei 1
  • Gholamreza Shahsavari 1
  • Fovad Abdolahpour 1
  • Shahrokh Bagheri 2
  • Marzeyeh Rashidi pour 3

1

2

3

چکیده [English]

Introduction: Oxidation of low-density lipoprotein (LDL) has been strongly implicated in the pathogenesis of atherosclerosis. The use of antioxidant compounds in dietary food stuff including vitamin E and herbal extract such as Walnut (Juglansregia L.) husks hydroalcoholic extract (W) may lead to inhibition of the production of oxidized LDL and may decrease both the development and the progression of atherosclerosis. The present study is an attempt to determine the effects of W on LDL oxidation induced-CuSO4 quantitatively in vitro. Material and Methods: Fasting blood samples were collected from normal people after an overnight fasting and then LDL was isolated. LDL was incubated without CuSO4 as control and with CuSO4 and several concentration of W (0.2, 2 and 20 µg/ml) the formation of conjugated dienes, lag time and Malondialdehyde (MDA)were measured. Inhibition of this Cu-induced oxidation was studied in the presence of several concentrations of W. Results: It was demonstrated that W is able to decrease CuSO4-induced LDL oxidation. Walnut (JuglansregiaL.) huske extract led to an increase rate of 87%, 178% and 202% lag time at concentrations ranging from 0.2µg/ml to 20 µg/ml, respectively. The inhibitory effects of W on LDL oxidation were dose-dependent at concentrations ranging from 0.2 µg/ml to 20 µg/ml. Conclusion: This study showed that W prevented the oxidation of LDL in vitro and it can be concluded that it might have similar effects in vivo.

کلیدواژه‌ها [English]

  • Walnut
  • LDL
  • In vitro
1. Willcox BJ, Curb JD, Rodriguez BL. Antioxidants in cardiovascular health and disease: key lessons from epidemiologic studies. Am J Cardiol 2008; 101(10A): 75D-86D. 2. Lobbes MB, Lutgens E, Heeneman S, et al. Is there more than C-reactive protein and fibrinogen? The prognostic value of soluble CD40 ligand, interleukin-6 and oxidized low-density lipoprotein with respect to coronary and cerebral vascular disease. Atherosclerosis 2006; 187(1): 18-25. 3. Steinberg D. Low density lipoprotein oxidation and its pathobiological significance. J Biol Chem 1997; 272(34): 20963-6. 4. Ani M, Moshtaghie AA, Ahmadvand H. Comparative Effects of Copper, Iron, Vanadium and Titanium on Low Density Lipoprotein Oxidation in vitro. Iran Biomed J 2007; 11(2): 113-18. 5. Witztum JL, Steinberg D. Role of oxidized low density lipoprotein in atherogenesis. J Clin Invest 1991; 88(6):1785-92. 6. Leopold JA, Loscalzo J. Oxidative risk for atherothrombotic cardiovascular disease. Free Radic Biol Med. 2009; 47(12): 1673-706. 7. Yekini I, Hammoudi F, Martin-Nizard F, Yous S, et al. Antioxidant activity of benzoxazolinonic and benzothiazolinonic derivatives in the LDL oxidation model. Bioorg Med Chem 2009; 17(22): 7823-30. 8. Pereira JA, Oliveira I, Sousa A, et al. Walnut (Juglans regia L.) leaves: Phenolic compounds, antibacterial activity and antioxidant potential of different cultivars. Food and Chem Toxicol 2007; 45(11): 2287-95. 9. McKay DL, Chen CY, Yeum KJ, et al. Chronic and acute effects of walnuts on antioxidant capacity and nutritional status in humans: a randomized, cross-over pilot study. Nutr J 2010; 9: 21. 10. Asgary S, Parkhideh S, Solhpour A, et al. Effect of ethanolic extract of Juglans regia L. on blood sugar in diabetes-induced rats. J Med Food 2008; 11(3): 533-8. 11. Stampar F, Solar A, Hudina M, et al. Traditional walnut liqueur – cocktail of phenolics. Food Chem 2006; 95(4): 627-31. 12. Liu J, Meng M, Li C, et al. Simultaneous determination of three diarylheptanoids and an α-tetralone derivative in the green walnut husks (Juglans regia L.) by high-performance liquid chromatography with photodiode array detector. J Chromatogr 2008; 1190(1-2): 80-5. 13. Carvalho M, Ferreira PJ, Mendes VS, et al. Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food Chem Toxicol 2010; 48(1): 441-7. 14. Bhatia K, Rahman S, Ali M, et al. In vitro antioxidant activity of Juglans regia L. bark extract and its protective effect on cyclophosphamide-induced urotoxicity in mice. Redox Rep 2006; 11(6): 273-9. 15. Crawford, RS, Mudaliar SR, Henry, et al. Inhibition of LDL oxidation in vitro but not ex vivo by troglitazone. Diabetes 1999; 48(4): 783-90. 16. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-54. 17. Navder KP, Baraona E, Leo MA, et al. Oxidation of LDL in baboons is increased by alcohol and attenuated by polyenylphosphatidylcholine. J Lipid Res 1999; 40(6): 983-7. 18. Seven A, Civelek S, Inci E, et al. Evaluation of oxidative stress parameters in blood of patients with laryngeal carcinoma. Clin Biochem 1999; 32(5): 369-73. 19. Ahmadvand H, Khosrobeigi A, Bagheri S, et al. Comparison of Inhibitory effects of Satureja Khozistanica oil extract, vitamin E and Coenzyme Q10 on LDL oxidation in vitro. Yafteh 2008; 11(4): 25-31. (Persian) 20. Lloyd DR, Phillips DH. Oxidative DNA damage mediated by copper(II), iron(II) and nickel(II) fenton reactions: evidence for site-specific mechanisms in the formation of double-strand breaks, 8-hydroxydeoxyguanosine and putative intrastrand cross-links. Mutat Res 1999; 424(1-2): 23-36. 21. Gutteridge JM. Inhibition of the Fenton reaction by the protein caeruloplasmin and other copper complexes. Assessment of ferroxidase and radical scavenging activities. Chem Biol Interact 1985; 56(1): 113-20. 22. Sheu JY, Chen PH, Tseng WC, et al. Spectrophotometric determination of a thiobarbituric acid-reactive substance in human hair. Anal Sci 2003; 19(6): 957-60. 23. Carvalho M, Ferreira PJ, Mendes VS, et al. Human cancer cell antiproliferative and antioxidant activities of Juglans regia L. Food and Chem Toxicol 2010; 48(1): 441-7. 24. Pereira JA, Oliveira I, Sousa A, et al. Bioactive properties and chemical composition of six walnut (Juglans regia L.) cultivars. Food Chem Toxicol. 2008; 46(6): 2103-11. 25. Almeida IF, Fernandes E, Jose LFC, et al. Walnut (Juglans regia) leaf extracts are strong scavengers of pro-oxidant reactive species. Food Chem 2008; 106(3): 1014-20. 26. Oliveira I, Sousa A, Ferreira IC, et al. Total phenols, antioxidant potential and antimicrobial activity of walnut (Juglans regia L.) green husks. Food Chem Toxicol 2008; 46(7): 2326-31.
مجله علوم پزشکی پارس
دوره 9، شماره 3 - شماره پیاپی 17
تیر 1390
صفحه 1-7