Evaluation of the expression hsa_circ_0043278 in renal cell carcinoma and its association with demographic and clinicopathological information of patients

Document Type : Original Article

Authors
Yaser Mansoori 1
Zahra firoozi 2
Narges majdodin 3
Elham Mohammadisoleimani 4
Seyed Alireza Dastgheib 5
Ali Ghanbari Asad 6
Behnam mansoori 7
Shirin Tavanafar 8
Mohammad Mehdi Naghizadeh 9
1 Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran ORCIDID: 0000-0002-9101-5552
2 PhD, Department of Medical Genetics, Fasa University of Medical Sciences, Fasa, Iran. ORCIDID: 0000-0002-8103-2244
3 MSc, Department of Medical Genetics, Shiraz University of Medical Sciences, Shiraz, Iran. ORCIDID:
4 MSc, Department of Medical Biotechnology, Fasa University of Medical Sciences, Fasa, Iran. ORCIDID: 0000-0002-3950-3102
5 MD. PhD, Department of Medical Genetics, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran;
6 Noncommunicable Diseases Research Center, Fasa University of Sciences, Fasa, Iran.
7 Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran
8 MD, Student Research Committee, Fasa University of Medical Sciences, Fasa, Iran.
9 PhD, Noncommunicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran. ORCIDID: 0000-0001-5562-103X
10.22034/pjms.2025.2068524.1226
Abstract
Abstract
Background and Objectives:
Renal cell carcinoma (RCC) is one of the most common urogenital tumors with high mortality. Increasing evidence suggests that circRNAs influence the malignant progression of various tumors. Previous studies have also revealed the role and function of various circRNAs in RCC. In this study, the role of hsa_circ_0043278 in renal cancer progression and its associated pathogenesis is investigated.
Materials and Methods:
After RNA extraction and cDNA synthesis, the expression level of hsa_circ_0043278 in RCC tumor samples was evaluated in comparison with normal adjacent tumor tissue using Real-time PCR. The association of hsa_circ_0043278 expression with clinicopathological and demographic information of patients was also investigated.
Results:
The results of our studies showed that the expression level of hsa_circ_0043278 in RCC tumor samples was significantly reduced compared to adjacent normal tissue. In addition, the analysis of the results showed that the expression of hsa_circ_0043278 was associated with a history of kidney disease in patients, such that in people with a history of kidney disease, the expression of this gene was lower.
Conclusion:
hsa_circ_0043278 is significantly reduced in RCC tumor cells and it is associated with a history of kidney disease. Therefore, it seems that hsa_circ_0043278, with its tumor suppressor role in renal carcinoma, plays a significant role in the signaling pathways involved in tumor development and progression.
Keywords
Renal cell carcinoma
hsa_circ_0043278
circRNAs
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