The effect of 8 weeks of HIIT training with calorie restriction of high-fat diet and standard diet on the expression of BDH1 and ACAT1 genes in the liver of healthy male Wistar rats

Document Type : Original Article

Authors
Omolbanin Ebrahim-Abadi 1
Soheil Amini-Zadeh 2
Daruosh Moflehi 3
1 . Master's Degree in Sports Physiology, Faculty of Physical Education and Sports Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
2 Assistant Professor, Department of Physiology and Pharmacology, Afzalipour School of Medicine and Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
3 Associate Professor at Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Shahid Bahonar University of Kerman, Kerman, Iran
10.22034/pjms.2024.2029968.1138
Abstract
 
Introduction: Calorie restriction is a nutritional intervention by reducing energy intake. The purpose of the current study was the effect of 8 weeks of HIIT training with calorie restriction of standard diet and high fat diet on the expression of BDH1 and ACAT1 genes in the liver of healthy male Wistar rats.
Materials & Methods: 48 male rats were randomly divided into 6 groups (n=8): control, exercise,calorie restriction of high-fat diet, calorie restriction of standard diet, calorie restriction of standard diet+ exercise, and calorie restriction of high-fat diet + exercise It was performed for 8 weeks (5 days a week) with an intensity of 100-90% of the maximum speed in the form of running on a treadmill with a zero-degree slope and in the morning (9 o'clock). Expression of BDH1 and ACAT1 genes in liver tissue was measured by Real Time-PCR method and one-way analysis of variance statistical method was used for comparison between groups.
Results: Exercise caused a significant increase in BDH1 gene expression compared to the control group (P<0.001). Simultaneous training with high-fat diet calorie restriction and standard diet calorie restriction led to a significant increase in BDH1 and ACAT1 gene expression compared to the control group (P<0.001).
Conclusion: In the conditions of calorie restriction, high-intensity interval training through the upregulation of BDH1 and ACAT1 gene expression may play a key role in increasing hepatic gluconeogenesis. Also, high-intensity interval training through the upregulation of the BDH1 gene can have play an important role in hepatic fat oxidation.
Keywords
ACAT1 gene
BDH1 gene
Calorie Restriction
Interval Training
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