Increasing radiation time in MCF-7 cancer cells and activating pathways related to type 1 diabetes mellitus
Document Type : Original Article
Authors
1 Department of Physiology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran
2 Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
3 Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran Department of Biochemistry and Nutrition, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran
Abstract
Introduction: Breast cancer is one of the most common cancers among women. Since microarray is a new method in
cancer diagnosis, in the current study, the microarray library of MCF-7 breast cancer cells after irradiation was examined to predict the gene expression pattern and metabolic pathways related to it.
Materials and Methods: Microarray data extracted from National Center for Biotechnology Information (NCBI) related to MCF- 7 cells were analyzed by GEO2R and R software. Then the genes that had increased and decreased expression were extracted and gene ontology analysis was performed using DAVID and Enrichr databases.
Results: In this study, after 48 hours of irradiation, 234 genes with Log2FC>1 increased expression and 146 genes with Log2FC<-1 decreased expression (Adjusted p value <0.05). KEGG analysis on genes with increased expression showed that these genes are related to type 1 diabetes (P=2.43E-04). The genes extracted in this pathway included HLA-DRB5, HLA-DRB4, CPE, TNF, HLA- DRB1 and HLA-DM (Adjusted p value <0.05). Also, the findings show that the genes involved in type 1 diabetes in the islets of Langerhans include CPE, MHC-II, and TNF-α.
Conclusion: The findings of this study showed that the increase in irradiation time in breast cancer cells probably
leads to the activation of pathways related to type 1 diabetes through CPE, MHC-II and TNF-α genes in the islets of Langerhans.
Keywords
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