Evaluation of lncRNA HPYR1 expression in colorectal cancer patients
Document Type : Original Article
Authors
Department of Biology, Science Faculty, Shahrekord Branch, Islamic Azad University, Shahrekord, Iran
Abstract
Introduction: Colon cancer is caused by abnormal growth of cells that can invade (Metastasis) or multiply in other tissues in the body. Colorectal cancer is the third most common cancer in the world and its mortality rate is almost 50% of patients. Research has shown that LncRNAs play an important role in tumorigenesis, angiogenesis, proliferation, migration, apoptosis and differentiation. Based on the above, the present study investigates the expression of LncRNA HPYR1 in patients with colorectal cancer.
Materials & Methods: The present study was performed by sampling 20 patients with colorectal cancer and 20 healthy tissues. Then RNA extraction was performed by Trizol kit and cDNA synthesis was performed by Takara kit and finally the expression of the relevant gene was performed by Real Time RT PCR technique. The relative expression level of LncRNA HPYR1 was calculated and finally the results were analyzed by GraphPad Prism software.
Results: The results showed that the expression of HPYR1 lncRNA in colorectal cancer has a significant increase compared to the control group (p=0.0116) and also the expression of the gene is related to metastasis (p=0.002). On the other hand, the relative expression of HPYR1 lncRNA in the tumor of people with stage 1 and 2 and in the tumor of people with different size tumors did not show a significant increase (p=0.56) and (p=0.8270), but the relative expression of lncRNA HPYR1 in the tumor of people with grades 2 had an increase in expression compared to grade 1 and it was statistically significant (p=0.03).
Conclusion: In the present study, the relative expression of LncRNA HPYR1 in people with colorectal cancer was increased compared to the control, which could be due to the activation of proteins involved in cancer and the growth of tumor cells and blood vessels. Birth in cancer. Therefore, considering the changes in LncRNA HPYR1 gene expression in colorectal cancer, it is possible to use this gene as a biomarker for tumor diagnosis.
Keywords
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