Effect of Hydroalcoholic Extract of Aloe Vera on Fetal Cerebellar Development in Streptozotocin-Induced Diabetic Rats

Document Type : Original Article

Authors
Mohammad Pourahmadi 1
Ehsan Rastgoo 2
Shekoufeh Atashpour 1
Arash Hasannezhad 2
Mohammad Aref Bagherzadeh 2
Hossein KargarJahromi 1
1 Research Center for Noncommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran
2 Student Research Committee, Jahrom University of Medical Sciences, Jahrom, Iran
10.22034/pjms.2023.707080
Abstract
Introduction: Diabetes mellitus has destructive effects on fetal brain development. Aloe vera extract contains potent
antioxidants which can be effective in treating diabetes. The present study investigated the effects of hydroalcoholic extract of Aloe vera on fetal cerebellar tissue in diabetic rats.
Materials and Methods: Forty eight adult pregnant Wistar rats were divided into 6 groups of 8 including control (C), receiving 400 mg/kg Aloe vera extract (CA), diabetic by injecting with 50 mg/kg streptozotocin (D), diabetic group receiving 20 IU/kg insulin (DI), diabetic group receiving 400 mg/kg Aloe vera extract (DA) and diabetic receiving 20 IU/kg insulin and 400 mg/kg Aloe vera extract (DAI). The rats received insulin subcutaneously and the Aloe vera extract through gavage. Blood glucose and body weight were recorded on days 1, 7, 14 and 18 of gestation. On day 18, the weight of each rat and the number and gender of its fetuses were recorded. Fetal brains were removed and tissue sections of their cerebellum were prepared. The results were analyzed through ANOVA and at the significant level of p≤0.05.
Results: Blood glucose in diabetic groups was significantly higher than C and A groups. Weight of pregnant rats on days 7,14, and 18 significantly decreased in the D, DI, and DAI groups compared to the C and A groups. In all diabetic groups compared to the C and A groups, the number of newborns significantly decreased and the neonatal weight increased. In group D, compared to group C, the thickness and number of gray and white matter cells, the thickness and number of molecular, Purkinje, and granular layer cells decreased significantly. Also, In DI and DAI groups compared to group D, the thickness of gray and white matter, the thickness and number of molecular layer cells and the number of white matter
cells showed a significant change compared to group D.  
Conclusion: It seems that aloe vera prevents the destruction of the fetus brain tissue of diabetic rats by reducing
blood sugar and preventing the increase of harmful oxidants.

 
Keywords
Diabetes mellitus
Aloe vera
Rat
fetus
Cerebellum
  • Sun J, Ren J, Hu X, Hou Y, Yang Y. Therapeutic effects of Chinese herbal medicines and their extracts on diabetes. Biomed Pharmacother 2021;142:111977.Nakamura U, Iwase M, Uchizono Y, Sonoki K, Sasaki N, Imoto H, Goto D, Iida M. Rapid intracellular acidification and cell death by H2O2 and alloxan in pancreatic β cells. Free Radic Biol Med 2006 1;40(11):2047-55.Bermejo‐Alvarez P, Roberts RM, Rosenfeld CS. Effect of glucose concentration during in vitro culture of mouse embryos on development to blastocyst, success of embryo transfer, and litter sex ratio. Mol Reprod Deve. 2012;79(5):329-36.Eriksson UJ, Cederberg J, Wentzel P. Congenital malformations in offspring of diabetic mothers—animal and human studies. Rev Endocr Metab Disord. 2003;4:79-93.Mills JL. Malformations in infants of diabetic mothers. Teratology 25:385-94. 1982. Birth Defects Res A Clin Mol Teratol. 2010;88(10):769-78.Hall JE, Hall ME. Guyton and Hall textbook of medical physiology e-Book. Elsevier Health Sciences; 2020: 13.Barthell JE, Georgieff MK. Infants of diabetic mothers. InNeonatology: A Practical Approach to Neonatal Diseases. Milano: Springer Milan. 2012: 379-86.Vinceti M, Malagoli C, Rothman KJ, Rodolfi R, Astolfi G, Calzolari E, Puccini A, Bertolotti M, Lunt M, Paterlini L, Martini M. Risk of birth defects associated with maternal pregestational diabetes. Europ J Epidemiol. 2014;29:411-8.Balsells M, García-Patterson A, Gich I, Corcoy R. Major congenital malformations in women with gestational diabetes mellitus: a systematic review and meta-analysis. Diabetes Metab Res Rev. 2012;28(3):252-7.Åberg A, Westbom L, Källén B. Congenital malformations among infants whose mothers had gestational diabetes or preexisting diabetes. Early Hum Deve. 2001 1;61(2):85-95.Buckner RL, Krienen FM, Castellanos A, Diaz JC, Yeo BT. The organization of the human cerebellum estimated by intrinsic functional connectivity. J Neurophysiol. 2011;106(5):2322-45.Gülcin I. Antioxidant activity of food constituents: an overview. Arch Toxicol. 2012;86:345-91.Isah AB, Ibrahim YK, Abdulrahman EM, Ibrahim MA. The hypoglycaemic activity of the aqueous extract of Stachytarpheta angustifolia (Verbanaceae) in normoglycaemic and alloxan-induced diabetic rats. Pakistan J Biological Sci. 2007 1;10(1):137-41Dhandapani S, Subramanian VR, Rajagopal S, Namasivayam N. Hypolipidemic effect of Cuminum cyminum L. on alloxan-induced diabetic rats. Pharmacol Res. 2002 1;46(3):251-5.Rajasekaran S, Sivagnanam K, Subramanian S. Antioxidant effect of Aloe vera gel extract in streptozotocin-induced diabetes in rats. Pharmacol Rep. 2005 1;57(1):90-6.Jadidoleslami M, Abbas Nejad M, Shahraki MR. The effect of Aloe vera aqueous extract on glucose and lipid levels in diabetic male rats. Iran J Diabet Metab. 2006 10;6(2):143-51.Taricco E, Radaelli T, Rossi G, Nobile de Santis MS, Bulfamante GP, Avagliano L, Cetin I. Effects of gestational diabetes on fetal oxygen and glucose levels in vivo. BJOG: Int J Obstet Gynaeco. 2009 Dec;116(13):1729-35.Bueno A, Sinzato YK, Volpato GT, Gallego FQ, Perecin F, Rodrigues T, Damasceno DC. Severity of prepregnancy diabetes on the fetal malformations and viability associated with early embryos in rats. Biol Reproduct. 2020;103(5):938-50.Majkutewicz I, Kurowska E, Podlacha M, Myślińska D, Grembecka B, Ruciński J, et al. Dimethyl fumarate attenuates intracerebroventricular streptozotocin-induced spatial memory impairment and hippocampal neurodegeneration in rats. Behav Brain Res. 2016 15;308:24-37Cobb CA, Cole MP. Oxidative and nitrative stress in neurodegeneration. Neurobiology Dis. 2015 1;84:4-21.Tesfaye S. Neuropathy in diabetes. Med. 2015 1;43(1):26-32.Zhao CH, Liu HQ, Cao R, Ji AL, Zhang L, Wang F, Yang RH. Effects of dietary fish oil on learning function and apoptosis of hippocampal pyramidal neurons in streptozotocin-diabetic rats. Brain Res. 2012 ;1457:33-43.Polster BM. AIF, reactive oxygen species, and neurodegeneration: a “complex” problem. Neurochem Int. 2013 Apr 1;62(5):695-702.Radha MH, Laxmipriya NP. Evaluation of biological properties and clinical effectiveness of Aloeávera: áAásystematic review. J Tradit Complement Med. 2015 1;5(1):21-6.Wang ZW, Zhou JM, Huang ZS, Yang AP, Liu ZC, Xia YF, et al. Aloe polysaccharides mediated radioprotective effect through the inhibition of apoptosis. J Radiat Res. 2004;45(3):447-54.Kosif R, Aktas RG, Oztekin A. The effects of oral administration of Aloe vera [barbadensis] on rat central nervous system: An experimental preliminary study. Neuroanat. 2008;7:22-7.