Plasmid-mediated quinolone resistance in Shigella sonnei and Shigella flexneri isolated from pediatric diarrheal

Authors
Mohsen Jafari 1
Laila Ghasemi Kia 2
Mohammadi Mohsen 3
Hossein Alimadadi 4
Arash Abbasi 5
Abazar Pournajaf 6
Ramin Kafshgari 7
Katayoun Borhani 8
Mahmoud Khodabandeh 8
1 Department of Pediatric Infectious Diseases, Bahrami Children Hospital, Tehran University of Medical Sciences, Tehran, Iran
2 Department of Microbiology, Faculty of Basic Science, Azad University of Borujerd, Borujerd, Iran
3 Non-Communicable Pediatric Diseases Research Center, Health Research Institute, Babol University of Medical Sciences, Babol, Iran
4 Pediatric Gastroenterology and Hepatology Research Center, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
5 Pediatric Chronic Kidney Disease Research Center, The Children's hospital Medical Center, Tehran University of Medical Sciences, Tehran, Iran
6 Department of Microbiology, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran
7 Student research committee, Babol University of medical sciences, Babol, Iran
8 Department of Pediatric Infectious Diseases, Pediatric's Center of Excellence, Children's Medical Center, Tehran University of Medical Sciences, Tehran, Iran
10.52547/jmj.17.1.8
Abstract
Introduction: Fluoroquinolones are one of the most common antibiotics in the treatment of Shigella infections
worldwide. The presence of plasmids carrying quinolone-resistance genes is one of the most important mechanisms for resistance to these drugs. Therefore, the aim of this study was to determine the presence of quinolone resistance plasmid genes (qnr) in Shigella sonnei and Shigella flexneri isolated from pediatric diarrhea.
Materials and methods: In a 10-month period from the beginning of June to the end of March 2012, 91 isolates of Shigella were obtained from 358 non-repetitive diarrhea samples. After identification and confirmation of Shigella
isolates, antibiotic susceptibility test was performed based on the CLSI instruction. The molecular PCR test was performed to amplify qnrA, qnrB, qnrC, qnrD and qnrS genes.
Results: Slide agglutination test showed that the prevalence of Shigella sonnei and Shigella flexneri were 69.2%
and 30.8%, respectively. The disc diffusion test showed that all Shigella flexneri were susceptible to levofloxacin. Most of the Shigella sonnei were resistant to Streptomycin. The frequency of qnrA, qnrB and qnrS genes were 26.4%, 74.7% and 46.2%, respectively. All strains were negative for the presence of qnrC and qnrD genes.
Conclusions: The qnrB gene is the most common plasmid-mediated quinolone resistance (PMQR) in our treatment
centers. Because of their plasmid origin, these genes have the ability to transfer and have high ability to spread to the other isolates. Since the change in pattern of antibiotic susceptibility is occurs, perform of antibiotic susceptibility test necessary.
Keywords
Quinolone
Plasmid
Shigella Sonnei
Shigella Flexneri
Children's Diarrhea
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