The effect of Saccharin on spermatogenes, testiscular tissue and pituitary – gonad hormones axis in rats

Authors
Reza Paknejad 1
Vahid Hemayatkhah Jahromi 2
1 Department of biology,Jahrom branch,Islamic Azad University ,Jahrom, Iran
2 Associated Proffessor, Department of biology,Jahrom branch ,Islamic Azad University,Jahrom,Iran
10.52547/jmj.16.4.19
Abstract
Introduction: Saccharin is an artificial, non-caloric sweetener used to sweeten products such as beverages, confectionery, biscuits, medicines and toothpastes. This is while our body is not able to metabolize it. In the present study, the effect of saccharin on spermatogenesis process, testicular tissue structure and hormonal axis of pituitary gonad in rats was investigated.
Materials and Methods: 35 adult male Wistar rats were classified into 5 equal groups in a completely randomized design. The control group did not receive any solvent or medicine. The control group received 1 ml distilled water per day as an intraperitoneal injection. Experimental groups received 1, 2 and 3 times daily, respectively, 250, 500 and 1000 mg / kg body weight saccharin intraperitoneal injection for 35 days, respectively. Blood samples were taken from animals after 35 days and testis tissue was isolated for histological examination. The obtained data were analyzed by SPSS software version 16 and one way ANOVA and Duncan's post hoc test.
Results: The amount of glucose in the experimental group was significantly higher than the control group (P≤0.05). The concentrations of testosterone, Dehydroepiandrosterone (DHEA), lutein and follicle stimulator in experimental groups have significantly reduced in comparison to the control group. Also, the number of Spermatogonium cells, primary and secondary spermatocytes, spermatid, spermatozoids and sperm in experimental groups have decreased significantly compared to the sham and control groups; but Sertoli and Leydig cells showed no any significant difference (P>0.05).
Conclusion: Saccharin can have a harmful effects on the rate of hormone and male sex cells, and the histology confirms the obtained hormonal results. Of course in this regard, the mode and dosage of using this common used artificial sweeter is very important.
Keywords
Saccharin
testis
Pituitary-Gonad hormones axis
Rat
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