Effect of Vero cell Co – culture on Quality of in vitro produced bovine blastocysts after vitrification

Authors
H Karimi Jashni
Mohammad Hossein Nasr esfhani
Hossein Emani
Mohammad Mardani
10.29252/jmj.3.3.1
Abstract
Introduction : Post freze – thaw embryo quality can be assessed by various methods , the most common being in vetro survival after a period of culture . several culture system have been used for this purpose and most of them include somatic cell such as oviduct , granulosa and  Buffalo rat liver cells as Co- culture support . The aim of this study was to assess the Vero cell Co- culture system on quality of in vitro produced bovine blastocysts after vitrification.


Materials & Methods :after in vitro maturation and fertilization of Immature bovine oocytes . presumptive zygotes were cultured in SoFand SoF medium for 7 days (39 c , 5% Co) . Then 100 blastorcysts were selected and vitrified  ( 40% ethylene glycol , 18% ficol , 0.3 mol sacurose).After tawing blastocysts were divided into two groups . One group was cultured in SOF : and the other group was cultured in SoF + Vero cell medium for 48 h. re-expanded blastocysts were selected, inner cell mass(ICM) from them were recovered by immunosurgery and stained cell with Hoechst .


Results : The mean number of ICM in SoF and SoF + Vero were 12 and 15 respectively . The percentage re-expanded blastocysts in SoFand SoF+ Vero were 42%,54% respectivety . there were significant difference between the number of ICM and percentage re–expanded blastocysts in the two groups.


Conclusion : Vero cell Co – culture system can improve viability of in vitro produced bovine blastosysts after vitrification . 
Keywords
Bovine blastocyst – Vero cell
Inner cell mas
Vitrification
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