Lack of association between HPV and sperm parameters as a risk factor in infertile men admitted to infertility clinic

Authors
Farzaneh Tafvizi
zahra tahmasebi fard
10.29252/jmj.13.3.37
Abstract
Introduction: Numerous factors contribute to male infertility including genital infections that may appear following microbial, fungal, and viral infections. Different studies have been recently conducted in the world on papillomavirus infection and its effect on functional parameters of sperm including motility, morphology, sperm count, and reduced male fertility. However, the correlation between papillomavirus infection and male infertility is still ambiguous. Materials and Methods: In this study, 50 semen samples of fertile men (as the control group) and 50 semen samples of infertile men were collected from Infertility Center of Qom Jihad Daneshgahi, Qom, Iran. The semen samples were analyzed according to World Health Organization’s standard methods and papillomavirus was detected using PCR and virus L1 region replication. Results: The analyzed spermogram of 50 infertile samples showed that 36%, 68%, and 72% of the samples had problems, respectively in terms of count, motility, and morphology, and 52% of the infertile samples had problems in all three parameters. The DNA of human papillomavirus was not detected in any fertile and infertile samples. Conclusion: Based on the results of this study, there was no correlation between papillomavirus infection and male infertility. It seems that, factors such as male circumcision, lack of homosexual behavior, and lack of multiple sexual partners were effective in reducing the prevalence of HPV in the studied male population. Keywords: Male infertility, papillomavirus, L1 gene
Keywords
Male infertility
Papillomavirus
L1 gene
1. Vosooghi S, Kheirkhah B, Kariminik A, et al. A review of the role of Mycoplasma infections in humans' infertility. NCMBJ 2012; 2(8): 9-20 [Persian]. 2.World Health Organization. Manual for the Standardized Investigation and Diagnosis of the Infertile Couple. Cambridge University Press, Cambridge, New York;1993. 3.Weidner W, Krause W, Ludwig M. Relevance of male accessory gland infection for subsequent fertility with special focus on prostatitis. Hum Reprod Update 1999; 5(5):421–432. 4. Fanaei H, Mardaneh J, Khayat S. An overview of the role of bacterial infection in male infertility. JFUMS 2013; 2(4): 227-234. 5. Foresta C, Garolla A, Zuccarello D, et al. Human papillomavirus found in sperm head of young adult males affects the progressive motility. J Fertil Steril 2010; 93(3):802-806. 6. Weyrich A. Preparation of Genomic DNA from Mammalian Sperm. Current protocols in Molecular Biology 2012;Chapter 2:Unit 2.13.1-3. 7. Shah KV, Daniel RW, Simons JW, et al. Investigation of colon cancers for human HPV genomic sequences by Polymerase Chain Reaction. J Surg Oncol 1992; 51(5):5-7. 8. Carrie M, Nilson M, Melody K, et al. Association between Male Anogenital HPV infection and Circumcision by Anatomic site sampled and lifetime Number of female sex partner. J Infect Dis 2009; 199(1):7-13. 9. Garolla A, Pizzol D, Bertoldo A, et al. Association, prevalence, and clearance of human papillomavirus and antisperm antibodies in infected semen samples from infertile patients. J Fertil Steril 2013; 99(1):125-131. 10. Bezold G, Politch JA, Kiviat NB, et al. Prevalence of sexually transmissible pathogens in semen from asymptomatic male infertility patients with and without leuko-cytospermia. J Fertil Steril 2007; 87(5):1087–1097. 11. Lai YM, Yang FP, Pao CC. Human papillomavirus deoxyribonucleic acid and ribonucleic acid in seminal plasma and sperm cells. J Fertil Steril1996; 65(5):1026-1030. 12. Lai YM, Lee JF, Huang HY, et al. The effect of human papillomavirus infection on sperm cell motility. J Fertil Steril 1997; 67(6):1152-1155. 13.Foresta C, Pizzol D, Moretti A, et al. Clinical and prognostic significance of human papillomavirus DNA in the sperm or exfoliated cells of infertile patients and subjects with risk factors. J Fertil Steril 2010; 94(5):1723-1727. 14. Garolla A, Pizzol D, Foresta C. The role of human papillomavirus on sperm function. Curr Opin Obstet Gynecol 2011; 23(4):232-237. 15. Yang Y, Jia CW, Ma YM, et al. Correlation between HPV sperm infection and male infertility. Asian J Androl 2013;15(4):529–532. 16. Cai T, Wagenlehner FM, Mondaini N,et al. Effect of human papillomavirus and Chlamydia trachomatis co-infection on sperm quality in young heterosexual men with chronic prostatitis-related symptoms. BJU Int 2014;113(2):281-287. 17. Tanaka H, Karube A, Kodama H, et al. Mass screening for human papillomavirus type 16 infection in infertile couples. J Reprod Med 2000; 45(11):907-911. 18. Foresta C, Pizzol D, Moretti A, et al. Clinical and prognostic significance of human papillomavirus DNA in the sperm or exfoliated cells of infertile patients and subjects with risk factors. J Fertil Steril 2010; 94(5):1723-1727. 19. Schillaci R, Capra G, Bellavia C, et al. Detection of oncogenic human papillomavirus genotypes on spermatozoa from male partners of infertile couples. J Fertil Steril 2013; 100(5):1236-1240. 20. Foresta C, Pizzol D, Bertoldo A, et al. Semen washing procedures do not eliminate human papilloma virus sperm infection in infertile patients. J Fertil Steril 2011; 96(5):1077-1082