The Effect of Memory Reinforcement by Cholinergic Agonist and Music on Creating Morphine Dependency in Mice Using Conditioned Place Preference (CPP) Method

Authors
Eebrahim Hosseini
Mokhtar Mokhtari
Akbar Vahdati
Neamat Razmi
Farnaz Tavakoli
10.29252/jmj.11.1.9
Abstract
The most important side effect of addictive drugs is their interaction with nervous system so that soon after taking the drug, the neurons̉ connections in nervous system are addicted to receiving the drugs frequently. The aim of this study was the effect of memory reinforcement by cholinergic agonist and music on creating morphine dependency. Materials and Methods: In this research, 110 male mature rats with the weight of 30-35grams were enrolled as the control, witness and experiment groups. The experimental group was included morphine, donepezil, morphine+donepezil, fast music, sedative music1, sedative music2, Morphine+ fast music, Morphine+ sedative music1 and Morphine+ sedative music2. Morphine was used to create dependency and donepezil was used as a memory reinforcement cholinergic factor. In order to estimate the level of dependency was used conditioned place preference (CPP) technique. Results: Results indicate a significant difference between control and morphine groups (p<0.05) and no significant difference among control group with groups of sham, donepezil and treated with music in the place preference. Also, significant difference between morphine group with groups of morphine+donepezil, morphine+fast music, morphine+sedative music1 and morphine+ sedative music2 in the in the place preference (p<0.05). Conclusion: Perhaps donepezil, the fast music and sedative music1 cause the reduction of Morphine-dependence CPP and sedative music2 increases Morphine-dependence CPP.
Keywords
Memory
Morphine
Donepezil
music
CPP