:: Volume 8, Issue 1 (spring 2010) ::
jmj 2010, 8(1): 7-13 Back to browse issues page
Nitric Oxide Production and Growth and Survival of Peritoneal Macrophages
Saeed Daneshmandi *, Monerah Hajimoradi, Maryam Roudbary, Afshin Amari
Abstract:   (3511 Views)


Toxoplasma gondii is an obligate intracellular parasite that invades a wide variety of host cells including macrophages and survives within it. The parts of Toxoplasma that participate in the mechanism of its evasion from immune system and macrophage defenses are not completely defined. In this study, we evaluated the effect of protein and DNA Toxoplasma fractions on proliferation and nitric oxide production by peritoneal macrophages.                                                                                   

Material and Methods:

The viability of macrophages was evaluated using3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium Bromide (MTT) reduction assay and the production of nitrite using Griess method.


MTT reduction and hence the growth and viability of macrophages in a dose of 200 ng/ml was significantly lower than those of the negative control (P=0.022); in other lower doses of protein it was not statistically significant (P>0.05). Different doses of Toxoplasma protein fraction did not affect NO production (P>0.05). MTT assay and NO production in different doses of DNA fraction was not different (P>0.05).                                                                                                                                      


According to the results of the present study, protein fraction of Toxoplasma has a suppressive effect on macrophage viability, but this effect is dose dependent. Protein fraction of Toxoplasma does not affect the amount of NO production by macrophage. The isolated DNA fraction of Toxoplasma did not influence the viability and NO production of macrophages. So, the ability of evasion of Toxoplasma gondii from macrophage defense is due to a component of its protein.                                                     

Keywords: Toxoplasma gondii, Macrophage, Nitric Oxide, MTT assay
Full-Text [PDF 193 kb]   (860 Downloads)    
Type of Study: Research | Subject: epidmilojgy
Received: 2016/05/25 | Revised: 2018/03/5 | Accepted: 2016/05/25

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Volume 8, Issue 1 (spring 2010) Back to browse issues page