:: Volume 14, Issue 3 (fall 2016) ::
jmj 2016, 14(3): 52-59 Back to browse issues page
Identification of Enterococci faecalis&E. faeaciumpathogens viaTehran hospitals clinical samples by phenotypic and genotypic methods and Evaluation of Antimicrobial Susceptibility in 2016
Sara Masoumi Zavaryani, Reza Mirnejd *, Shohreh Zareh, Vahhab Piranfar, Ozra Bagheri Bejestani
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Abstract:   (11031 Views)

Background: Nowadays, the epidemiology of enterococcal infections has attracted a lot of attention. This led to significant changes occur in the field. Also identification of Enterococcus is important and can be effective to the control flow microorganism antibiotics resistance. So the aim of this study was Identification of Enterococci faecalis & E. faeacium pathogens via Tehran hospitals clinical samples by phenotypic and genotypic methods and evaluation of antimicrobial susceptibility pattern in 2016.

Methods: This study was performed on 400 clinical samples from different hospitals in Tehran, Iran in 2015-2016.Specific cultures were used for identification of Enterococcus. Biochemical test was used to recognize E.faecalis and E.faeacium specious. Then, PCR method was used to identify Enterococcus spp. Amplified DNA sequenced to assure reproduction. Antibiotic susceptibility test was done by disc diffusion method. 

Results: By phenotypic method, 278 Enterococcus were recognized from 400 samples, in which 70.86% were Enterococcus faecalis strains, 15.46% E. faeacium strains and 13.66% were other strains of Enterococcus species. While by using genotypic methods based on PCR, 72.3% were recognized E. faecalis strains, 10.43% E.faeacium strains and 17.62% were strains of other Enterococcus species. Results of antibiograms pattern showed gentamicin was most resistant and least resistant was linezolid. 9 cases were resistant to vancomycin MIC ≥ 512 µg/ml.

Conclusion: Rapid detection can be useful to prevent a massive outbreak of Enterococcus. Also according to the prevalence of vancomycin resistant enterococcus which was shown, the necessity of preventive measures reported. Due to resistance patterns, in order to prescribe the right antibiotics, anti-susceptibility testing for each patient before treatment is recommended.


Keywords: Enterococcus faecalis, Enterococcus faecium, Phenotypic method, PCR, Molecular detection
Full-Text [PDF 421 kb]   (2366 Downloads)    
Type of Study: Research | Subject: Microbshensi
Received: 2016/04/24 | Revised: 2017/06/12 | Accepted: 2016/09/6

XML   Persian Abstract   Print

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Volume 14, Issue 3 (fall 2016) Back to browse issues page