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:: Volume 14, Issue 2 (summer 2016) ::
jmj 2016, 14(2): 59-67 Back to browse issues page
Investigation Cytotoxic Effect of Hydroalcholic Extract from Combination of Kelussia odaratissma Mozaff and Thymus daenesis Celak on MCF-7 Cancer Cells Line
Fatemeh Sadeghisamani, Hosein Sazgar *, Abdollah Ghasemi pirbalouti
Islamic Azad University, Sahrekord, Iran
Abstract:   (4882 Views)

Introduction: Breast cancer is the second common cancer after lung cancer in women. Herbal preparations are used to treat centuries. The aim of this study was cytotoxic effect hydroalcholic extract from Combinatiin of Kelussia odaratissma Mozaff and Thymus daenesis Celak on MCF-7 cancer cells line.

Mateerial and method: MCF-7 cancer cells and Fibroblast normal cells were cultured in DMEM medium contaninig with Fetal Bovine Serum (FBS) and Antibiotic.The cells exposed to different doses of hydroalcholic extract from Combination of Kelussia odaratissma Mozaff and Thymus daenesis Celak (0/156, 0/312, 0/625, 1/25, 2/5 mg/ml) and were incubated for 24, 48 and 72 hours. After the incubation period, modifid colorimetric MTT method was used to determine the cellular toxicity of the extract.

Results: The results of the MTT test showed that Hydroalcholic extract from Combinatiin of Kelussia odaratissma Mozaff and Thymus daenesis Celak has dose-dependedent and time-dependent anti cancer effect on MCF-7 cancer cells. The highest percentage of cell death was observed after 72 h incubation and increase in extract concentration(p< 0/05). extract did not show significant cytotoxicity on Fibroblast  normal cells.

Discussion and Conclusion: Hydroalcholic extract from Combinatiin of Kelussia odaratissma Mozaff and Thymus daenesis Celak has cytotoxic effect on MCF-7 cancer cells, However this extract does not have any cytotoxic effect on Fibroblast  normal cells. It seems to come with further research, and utilizes its are compound in cancer treatment.

Keywords: Kelussia odaratissma Mozaff, Thymus daenesis Celak, MCF-7, MTT assay
Full-Text [PDF 312 kb]   (2987 Downloads)    
Type of Study: Research | Subject: Physiology
Received: 2016/04/18 | Revised: 2017/06/12 | Accepted: 2016/08/7
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Sadeghisamani F, Sazgar H, Ghasemi pirbalouti A. Investigation Cytotoxic Effect of Hydroalcholic Extract from Combination of Kelussia odaratissma Mozaff and Thymus daenesis Celak on MCF-7 Cancer Cells Line. jmj. 2016; 14 (2) :59-67
URL: http://jmj.jums.ac.ir/article-1-695-en.html


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Volume 14, Issue 2 (summer 2016) Back to browse issues page
فصلنامه دانشگاه علوم پزشکی جهرم Journal of Jahrom University of Medical Sciences
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