:: Volume 11, Issue 2 (summer2013 2013) ::
jmj 2013, 11(2): 31-38 Back to browse issues page
Transdifferentiation of human synovium-derived mesenchymal stem cell into neuronal-like cells in vitro
Elham Kashafi, Hojat Karimi Jashni *, Saedeh Erfaniyan, Kavos Solhjou, Ali Sepidkar, Hesam Fakhryniya
Abstract:   (12687 Views)
Introduction: Synovial mesenchymal stem cells (SMSCs) could be expanded extensively in monolayer with limited senescence, maintaining their multilineage differentiation potential in vitro. We characterized the multi-potent ability of human synovial membrane derived stem cells (SMSCs) and investigated the neural differentiation potential of these cells. Materials and Methods: SMSCs were isolated from the knee joint of ACL (Anterior cruciate ligament) and baker cyst patient. These cells were proliferated and amplified with the indicated concentration of FBS (Fetal Bovin Serum) and DMEM in vitro. Alizarin red and oil red staining was done to investigate stemness property of the cells. Then, they were induced in vitro by β-ercaptoethanol (BME), and Retinoic acid (RA). Reverse transcription polymerase chain reaction (RT-PCR) analysis was done to prove the neural gene and osteocyte and adipocyte cells specific genes. Results: RT-PCR analysis showed the expression of osteogenic and adipogenic genes. Following neural induction, SMSCs were differentiated into various types of neural cells in vitro. RT-PCR analysis also demonstrated that the mRNA levels encoding for neurofilament medium (NFM), neuron specific enolase (NSE) were also highly increased in induced SMSCs. Conclusion: These results suggest that synovium tissue, which is discarded in most of the knee operations, can be used for cell therapy and tissue engineering protocols as an enrichment source of potent mesenchymal stem cells. Induction of SMSCs by inducers such as BME-RA could highly transdifferentiate SMSCs into neuronal-like cells.
Keywords: Stem Cells, Cell Differentiation, Synovium
Full-Text [PDF 531 kb]   (2920 Downloads)    
Type of Study: Research |
Received: 2013/08/27 | Revised: 2018/03/3 | Accepted: 2013/09/23



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Volume 11, Issue 2 (summer2013 2013) Back to browse issues page