: The present study was designed for determination of the pattern of antibiotic resistance of Escherichia coli (E. coli) isolated from urinary tract infection in Jahrom in aim of rapid and appropriate empirical management .Method: A cross sectional study was carried out on 108 urine samples of patients with urinary tract infection in Jahrom hospitals and general practice in 2003. Antibiotic susceptibility was done by disk diffusion method and MIC. Result: Gentamicin had at least effect on E. coli with 72.1% resistance. Co-trimoxazol and rifampin were the most effective oral antibiotics. 89.5 percent of E.coli were resistant to two or more antibiotics. The most associated resistance was co-trimoxazol- rifampin and cotrimoxazol- gentamicin. Conclusion: Based on this data gentamicin and co-trimoxazol were less effective for treatment of urinary tract infection, but nalidixic acid and ciprofloxacin were appropriate for empirical treatment