Jahrom University of Medical SciencesPars Journal of Medical Sciences2717-028414320221225The effect of cinnamon on the Elimination of lead poisoning on sex hormone changes in adult female ratsThe effect of cinnamon on the Elimination of lead poisoning on sex hormone changes in adult female rats1770033310.29252/jmj.14.3.1FAParvinBagherzadehJournal Article20221225Introduction: Lead is one of the toxic and heavy metals that had different effects and side effects on biological systems of living creatures suchas humon, also, it causes various risks such as infertility. This study investigate antioxidant effect of cinnamon on elimination of lead poisoning in reproductive system in order to find a solutim for the problem of people in facing with lead.
Materials and methods: The animals in this into 7 groups of 6 rats that includes one control group which received no remedy, control group 1 (solvent), control group 2 (0.6 g/l lead acetate daily), control group 3 (1.5 g/kg cinnamon extract daily), experimental group 1,2,3 that daily received 0.6 g/l lead acetate in addition to respectively received 0.1, 0.2 , 0.5 g/kg cinnamon extract for 14 days. Then, statistical data determineal significantly by ANOVA (one – way analysis of variance) through SPSS software , version 18.
Results: The results shows that The concentration of the hormone estrogen decreased in control group 2 and increased in control group 3 signiticantly compared to control group in 5% level. The concentrate of hormone progesterone decreased in control group 2 and increased in control group 3 significantly compared to control group.
Conclusion: The results of this study indicates that cinnamon extract in a dose dependent manner and with antioxidant properties reduces the side effects of lead on sex hormones.Introduction: Lead is one of the toxic and heavy metals that had different effects and side effects on biological systems of living creatures suchas humon, also, it causes various risks such as infertility. This study investigate antioxidant effect of cinnamon on elimination of lead poisoning in reproductive system in order to find a solutim for the problem of people in facing with lead.
Materials and methods: The animals in this into 7 groups of 6 rats that includes one control group which received no remedy, control group 1 (solvent), control group 2 (0.6 g/l lead acetate daily), control group 3 (1.5 g/kg cinnamon extract daily), experimental group 1,2,3 that daily received 0.6 g/l lead acetate in addition to respectively received 0.1, 0.2 , 0.5 g/kg cinnamon extract for 14 days. Then, statistical data determineal significantly by ANOVA (one – way analysis of variance) through SPSS software , version 18.
Results: The results shows that The concentration of the hormone estrogen decreased in control group 2 and increased in control group 3 signiticantly compared to control group in 5% level. The concentrate of hormone progesterone decreased in control group 2 and increased in control group 3 significantly compared to control group.
Conclusion: The results of this study indicates that cinnamon extract in a dose dependent manner and with antioxidant properties reduces the side effects of lead on sex hormones.https://jmj.jums.ac.ir/article_700333_9519f2e350648d39221a07df7b42749c.pdfJahrom University of Medical SciencesPars Journal of Medical Sciences2717-028414320221225The frequency of qacA/B and smr genes in clinical isolates of methicillin resistance coagulase negative staphylococciThe frequency of qacA/B and smr genes in clinical isolates of methicillin resistance coagulase negative staphylococci81770033410.29252/jmj.14.3.8FAMohammadBokaeianJavadAdabiHamedTahmasebiJournal Article20221225Introduction:
Increasing use of disinfectants biocide cause to appearance of resistant strains of coagulase negative Staphylococcus. Some research confirmed this gene responsible for resistance to methicillin and association with these agents. The aim of this study was to investigate the presence of resistance genes of biocides such as qac A/B and smr in coagulase negative staphylococci.
Materials & Methods:
In this cross-sectional study, 60 samples of Staphylococcus epidermidis and 49 samples of Staphylococcus saprophyticus collected over a period of 9 months from clinical samples. After the initial biochemical tests and confirmed genus and species isolates, using specific primers for aqcA / B and smr genes studied by using polymerase chain reaction(PCR) method. Data were analyzed using chi-square test
Results:
Of 60 isolates of Staphylococcus saprophyticus, 36 isolates had mecA gene. Among these, significantly 19 isolates qacA gen and 21 isolated smr gene. also of 49 Staphylococcus epidermidis isolates, 27 isolates were mecA gene and among those isolates ,11 isolates have gene qacA / B and 8 isolate have smr genes.
Conclusions:
The results of this study, we observed the wide spread presence of genes qac A/B and smr in clinical isolates of methicillin-resistant coagulase-negative staphylococci. Due to the prevalence methicillin-resistant strains obtained in isolation,Introduction:
Increasing use of disinfectants biocide cause to appearance of resistant strains of coagulase negative Staphylococcus. Some research confirmed this gene responsible for resistance to methicillin and association with these agents. The aim of this study was to investigate the presence of resistance genes of biocides such as qac A/B and smr in coagulase negative staphylococci.
Materials & Methods:
In this cross-sectional study, 60 samples of Staphylococcus epidermidis and 49 samples of Staphylococcus saprophyticus collected over a period of 9 months from clinical samples. After the initial biochemical tests and confirmed genus and species isolates, using specific primers for aqcA / B and smr genes studied by using polymerase chain reaction(PCR) method. Data were analyzed using chi-square test
Results:
Of 60 isolates of Staphylococcus saprophyticus, 36 isolates had mecA gene. Among these, significantly 19 isolates qacA gen and 21 isolated smr gene. also of 49 Staphylococcus epidermidis isolates, 27 isolates were mecA gene and among those isolates ,11 isolates have gene qacA / B and 8 isolate have smr genes.
Conclusions:
The results of this study, we observed the wide spread presence of genes qac A/B and smr in clinical isolates of methicillin-resistant coagulase-negative staphylococci. Due to the prevalence methicillin-resistant strains obtained in isolation,https://jmj.jums.ac.ir/article_700334_de01927f8c2c4ad9dcc034c327ac8de1.pdfJahrom University of Medical SciencesPars Journal of Medical Sciences2717-028414320221225Analgesic effect of chlorpheniramine in rat anaesthesia with acepromazine â ketamine combinationAnalgesic effect of chlorpheniramine in rat anaesthesia with acepromazine â ketamine combination182670033510.29252/jmj.14.3.18FAShahinHajighahramaniAliMojtahedinVahidGharib KhajehJournal Article20221225
Background & Objective: Recently, antihistamines is known as analgesic agents. The aim of the present study is to investigate the analgesic activity of chlorpheniramine (H1 antagonist), during anesthesia with acepromazine- ketamine combination and surgical operation in rat.
Methods & materials: 20 adult rats were randomly assigned in two groups. In first group, chlorpheniramine was administered and 30 minutes later acepromazine – Ketamine was injected. In second group, Normal saline was administerd initially and then acepromazine - Ketamine combination was injected. Assessment of pain reflexes was performed by toe pinch , tail pinch test and reflex of rats to laparotomy operation during anesthesia. Induction time of anesthesia, duration of light anesthesia, surgical anesthesia time, walking time, heart rate and respiratory rate was measured.
Results: Time of light anesthesia in group 2 was shorter than group 1 (P < 0.05). Laparotomy pain score was significantly different in first group and second group. Toe pinch and tail reflex didn’t show significant differences between two groups. Heart rate in all rats not showed significant differences in compared together .
Conclusion: chlorpheniramine as a premedication was induced appropriate balance anesthesia in rat with acepromazine- ketamine combination. Thus, chlorpheniramine could be used in rat as premedication agent for surgical operations in combined with acepromazine- ketamine.
Background & Objective: Recently, antihistamines is known as analgesic agents. The aim of the present study is to investigate the analgesic activity of chlorpheniramine (H1 antagonist), during anesthesia with acepromazine- ketamine combination and surgical operation in rat.
Methods & materials: 20 adult rats were randomly assigned in two groups. In first group, chlorpheniramine was administered and 30 minutes later acepromazine – Ketamine was injected. In second group, Normal saline was administerd initially and then acepromazine - Ketamine combination was injected. Assessment of pain reflexes was performed by toe pinch , tail pinch test and reflex of rats to laparotomy operation during anesthesia. Induction time of anesthesia, duration of light anesthesia, surgical anesthesia time, walking time, heart rate and respiratory rate was measured.
Results: Time of light anesthesia in group 2 was shorter than group 1 (P < 0.05). Laparotomy pain score was significantly different in first group and second group. Toe pinch and tail reflex didn’t show significant differences between two groups. Heart rate in all rats not showed significant differences in compared together .
Conclusion: chlorpheniramine as a premedication was induced appropriate balance anesthesia in rat with acepromazine- ketamine combination. Thus, chlorpheniramine could be used in rat as premedication agent for surgical operations in combined with acepromazine- ketamine. https://jmj.jums.ac.ir/article_700335_6deabbd1859b0508571345a3869c1d25.pdfJahrom University of Medical SciencesPars Journal of Medical Sciences2717-028414320221225The Effect of resistance training circuit with Medicago sativa extracts on levels of Osteoprotegerin and Nuclear Factor of Kappa-B in thin girlsThe Effect of resistance training circuit with Medicago sativa extracts on levels of Osteoprotegerin and Nuclear Factor of Kappa-B in thin girls273470033610.29252/jmj.14.3.27FAParvinFarzanegiKhaterehEbrahimiMasoumehHabibian0000-0003-1028-1726Journal Article20221225Abstract
Introduction:
Osteoporosis is the most common metabolic bone disease with high prevalence that various factors are involved in the pathogenesis of this disease, including the low body mass index and lack of physical activity. Change in lifestyle represents a successful strategy to prevent future disease. Therefore the aim of this study was determine the interactive effects of resistance training and Medicago sativa extracts consumption on serum level of steoprotegerin and nuclear factor kappa B in thin girls.
Materials and Methods:
In one Quasi-experimental study, 28 female students (16 to 19 years) divided into 4 groups, including: supplement, training , training-supplement and Control randomly. Resistance training program consisted of 10 exercise movements at 60-80% of one repeated maximal in 8-12 repetition (in 3 circuits), 60 min per day and 3 sessions per week for 4 weeks. Intake Medicago sativa supplement was 1 ml/kg body weight for 3 times a day. Data were analyzed with Paired t and ANOVA tests ( P<0.05) .
Results:
Four weeks of resistance training, supplementation and the combined intervention were associated with a significant increase in steoprotegerin and decrease in nuclear factor kappa B levels (P<0/05) in thin girls. Furthermore combined intervention were associated with greater changes in percent changes of these inducator compare training and supplement groups (p<0.05).
Conclusion:
Regular resistance training with consumption of Medicago sativa extracts as a non-drug therapy can increase bone formation markers and also reduce the activity of the absorbency pathway in thin adolescent girls. Of course the combination of exercise and supplement are more effective than the separate effects of each them.Abstract
Introduction:
Osteoporosis is the most common metabolic bone disease with high prevalence that various factors are involved in the pathogenesis of this disease, including the low body mass index and lack of physical activity. Change in lifestyle represents a successful strategy to prevent future disease. Therefore the aim of this study was determine the interactive effects of resistance training and Medicago sativa extracts consumption on serum level of steoprotegerin and nuclear factor kappa B in thin girls.
Materials and Methods:
In one Quasi-experimental study, 28 female students (16 to 19 years) divided into 4 groups, including: supplement, training , training-supplement and Control randomly. Resistance training program consisted of 10 exercise movements at 60-80% of one repeated maximal in 8-12 repetition (in 3 circuits), 60 min per day and 3 sessions per week for 4 weeks. Intake Medicago sativa supplement was 1 ml/kg body weight for 3 times a day. Data were analyzed with Paired t and ANOVA tests ( P<0.05) .
Results:
Four weeks of resistance training, supplementation and the combined intervention were associated with a significant increase in steoprotegerin and decrease in nuclear factor kappa B levels (P<0/05) in thin girls. Furthermore combined intervention were associated with greater changes in percent changes of these inducator compare training and supplement groups (p<0.05).
Conclusion:
Regular resistance training with consumption of Medicago sativa extracts as a non-drug therapy can increase bone formation markers and also reduce the activity of the absorbency pathway in thin adolescent girls. Of course the combination of exercise and supplement are more effective than the separate effects of each them.https://jmj.jums.ac.ir/article_700336_8acbeba7a1683d48700e1e8afef7f7b0.pdfJahrom University of Medical SciencesPars Journal of Medical Sciences2717-028414320221225Isolation and culture of mouse newborn skin fibroblast cells in a simple and inexpensive methodIsolation and culture of mouse newborn skin fibroblast cells in a simple and inexpensive method354270033710.29252/jmj.14.3.35FAAlirezaKheirollahMostafaChashmpooshAsmaMohammadiNedaAbdovisHamideGhasempoorJournal Article20221225Background and objective
Cell culture is one of the main techniques used in scientific researches that done in three ways: Primary culture, secondary culture or cell passage and purchase Cell Lines of Cell banks. Fibroblast cell is an important component of connective tissue that is found almost everywhere in the body. Fibroblast cells based on their location have functional and morphology roles. The aim of this project was simplification through innovation and localization, and to obtain mouse newborn skin fibroblast cells in order to use these cells for further study.
Methods
For this project were used from mouse neonatal that is one to two days. Initially all devices, including surgical materials, culture dishes, buffer and etc were sterilized by autoclaving at 121 ° C and intense pressure. As well as workplace were sterilized by 70% alcohol and UV. Initially mouse neonatal under the biology hood and in 70% alcohol were killed and were sterilized with sterile instruments and then skin fibroblast cells were isolated from these mice. In addition skin fibroblast cells for further growth were transferred of the primary environment to a fresh and new medium and first passages was performed on these cells.
Results
Fibroblast cells in different days were evaluated in terms of growth and morphology by fluorescent microscope (Olympus) with a magnification of 10, that this cells in terms of morphology were appropriate.
Conclusion
According to the results of this study, the simplicity of this method, no need to high costs and sterile conditions without the use of antibiotics and anti-fungal, makes reasonable use of this method for isolation and cell culture in different studies.
Keywords: Cell culture, Fibroblast cells, Mouse newbornBackground and objective
Cell culture is one of the main techniques used in scientific researches that done in three ways: Primary culture, secondary culture or cell passage and purchase Cell Lines of Cell banks. Fibroblast cell is an important component of connective tissue that is found almost everywhere in the body. Fibroblast cells based on their location have functional and morphology roles. The aim of this project was simplification through innovation and localization, and to obtain mouse newborn skin fibroblast cells in order to use these cells for further study.
Methods
For this project were used from mouse neonatal that is one to two days. Initially all devices, including surgical materials, culture dishes, buffer and etc were sterilized by autoclaving at 121 ° C and intense pressure. As well as workplace were sterilized by 70% alcohol and UV. Initially mouse neonatal under the biology hood and in 70% alcohol were killed and were sterilized with sterile instruments and then skin fibroblast cells were isolated from these mice. In addition skin fibroblast cells for further growth were transferred of the primary environment to a fresh and new medium and first passages was performed on these cells.
Results
Fibroblast cells in different days were evaluated in terms of growth and morphology by fluorescent microscope (Olympus) with a magnification of 10, that this cells in terms of morphology were appropriate.
Conclusion
According to the results of this study, the simplicity of this method, no need to high costs and sterile conditions without the use of antibiotics and anti-fungal, makes reasonable use of this method for isolation and cell culture in different studies.
Keywords: Cell culture, Fibroblast cells, Mouse newbornhttps://jmj.jums.ac.ir/article_700337_f9060d27f13dd865265e4fc48e16f726.pdfJahrom University of Medical SciencesPars Journal of Medical Sciences2717-028414320221225Comparison of direct PCR and PCR using DNA extracted kit for determine tst and mecA genes in Staphylococcus aureusComparison of direct PCR and PCR using DNA extracted kit for determine tst and mecA genes in Staphylococcus aureus435170033810.29252/jmj.14.3.43FAHamedTahmasebiMohammadBokaeianMojdehJahantighJavadAdabiJournal Article20221225Abstract
Introduction: PCR reactions one of the most accurate and most sensitive tests for the molecular assays. DNA extraction always is time-consuming and costly before the PCR process, which in some cases removed can be saving time and Price. The aim of this study was to compare direct PCR and PCR with DNA extraction template for detection of tst, mecA and femA gens.
Materials and Methods: In this study, clinical isolates of Staphylococcus aureus were confirmed by biochemical tests. Then, with direct PCR by colony acquired of Muller Hinton agar culture and PCR with the extracted DNA extraction kit were studied for tst and mecA genes. For this test, three specific primers were used to target genes.
Findings: amplification was observed of tst, mecA and femA gens with length 326bp, 163bp and 132bp by direct PCR and PCR with the extracted DNA relevant to toxic shock syndrome toxin 1 and are resistant to methicillin. Finally, detected resistant strains and toxins strains by genes. Our results quantitatively Similar each other and in therm of qualitatively were different. The results of PCR test with using extraction kit obtained the better quality.
Conclusions: According to the results obtained in this study, to save time and cost in the detection of Staphylococcus aureus can be used to direct PCR. Bands quality obtained in this way to PCR, DNA, is acceptable and in some cases it can be used as a rapid diagnostic test. To make the better quality must be used mineral oils.Abstract
Introduction: PCR reactions one of the most accurate and most sensitive tests for the molecular assays. DNA extraction always is time-consuming and costly before the PCR process, which in some cases removed can be saving time and Price. The aim of this study was to compare direct PCR and PCR with DNA extraction template for detection of tst, mecA and femA gens.
Materials and Methods: In this study, clinical isolates of Staphylococcus aureus were confirmed by biochemical tests. Then, with direct PCR by colony acquired of Muller Hinton agar culture and PCR with the extracted DNA extraction kit were studied for tst and mecA genes. For this test, three specific primers were used to target genes.
Findings: amplification was observed of tst, mecA and femA gens with length 326bp, 163bp and 132bp by direct PCR and PCR with the extracted DNA relevant to toxic shock syndrome toxin 1 and are resistant to methicillin. Finally, detected resistant strains and toxins strains by genes. Our results quantitatively Similar each other and in therm of qualitatively were different. The results of PCR test with using extraction kit obtained the better quality.
Conclusions: According to the results obtained in this study, to save time and cost in the detection of Staphylococcus aureus can be used to direct PCR. Bands quality obtained in this way to PCR, DNA, is acceptable and in some cases it can be used as a rapid diagnostic test. To make the better quality must be used mineral oils.https://jmj.jums.ac.ir/article_700338_3eedbc7b7e652fa0d2ce746ab08a0fcb.pdfJahrom University of Medical SciencesPars Journal of Medical Sciences2717-028414320221225Identification of Enterococci faecalis&E. faeaciumpathogens viaTehran hospitals clinical samples by phenotypic and genotypic methods and Evaluation of Antimicrobial Susceptibility in 2016Identification of Enterococci faecalis&E. faeaciumpathogens viaTehran hospitals clinical samples by phenotypic and genotypic methods and Evaluation of Antimicrobial Susceptibility in 2016525970033910.29252/jmj.14.3.52FASaraMasoumi ZavaryaniRezaMirnejdShohrehZarehVahhabPiranfarOzraBagheri BejestaniJournal Article20221225Background: Nowadays, the epidemiology of enterococcal infections has attracted a lot of attention. This led to significant changes occur in the field. Also identification of Enterococcus is important and can be effective to the control flow microorganism antibiotics resistance. So the aim of this study was Identification of Enterococci faecalis & E. faeacium pathogens via Tehran hospitals clinical samples by phenotypic and genotypic methods and evaluation of antimicrobial susceptibility pattern in 2016.
Methods: This study was performed on 400 clinical samples from different hospitals in Tehran, Iran in 2015-2016.Specific cultures were used for identification of Enterococcus. Biochemical test was used to recognize E.faecalis and E.faeacium specious. Then, PCR method was used to identify Enterococcus spp. Amplified DNA sequenced to assure reproduction. Antibiotic susceptibility test was done by disc diffusion method.
Results: By phenotypic method, 278 Enterococcus were recognized from 400 samples, in which 70.86% were Enterococcus faecalis strains, 15.46% E. faeacium strains and 13.66% were other strains of Enterococcus species. While by using genotypic methods based on PCR, 72.3% were recognized E. faecalis strains, 10.43% E.faeacium strains and 17.62% were strains of other Enterococcus species. Results of antibiograms pattern showed gentamicin was most resistant and least resistant was linezolid. 9 cases were resistant to vancomycin MIC ≥ 512 µg/ml.
Conclusion: Rapid detection can be useful to prevent a massive outbreak of Enterococcus. Also according to the prevalence of vancomycin resistant enterococcus which was shown, the necessity of preventive measures reported. Due to resistance patterns, in order to prescribe the right antibiotics, anti-susceptibility testing for each patient before treatment is recommended.
Keywords: Background: Nowadays, the epidemiology of enterococcal infections has attracted a lot of attention. This led to significant changes occur in the field. Also identification of Enterococcus is important and can be effective to the control flow microorganism antibiotics resistance. So the aim of this study was Identification of Enterococci faecalis & E. faeacium pathogens via Tehran hospitals clinical samples by phenotypic and genotypic methods and evaluation of antimicrobial susceptibility pattern in 2016.
Methods: This study was performed on 400 clinical samples from different hospitals in Tehran, Iran in 2015-2016.Specific cultures were used for identification of Enterococcus. Biochemical test was used to recognize E.faecalis and E.faeacium specious. Then, PCR method was used to identify Enterococcus spp. Amplified DNA sequenced to assure reproduction. Antibiotic susceptibility test was done by disc diffusion method.
Results: By phenotypic method, 278 Enterococcus were recognized from 400 samples, in which 70.86% were Enterococcus faecalis strains, 15.46% E. faeacium strains and 13.66% were other strains of Enterococcus species. While by using genotypic methods based on PCR, 72.3% were recognized E. faecalis strains, 10.43% E.faeacium strains and 17.62% were strains of other Enterococcus species. Results of antibiograms pattern showed gentamicin was most resistant and least resistant was linezolid. 9 cases were resistant to vancomycin MIC ≥ 512 µg/ml.
Conclusion: Rapid detection can be useful to prevent a massive outbreak of Enterococcus. Also according to the prevalence of vancomycin resistant enterococcus which was shown, the necessity of preventive measures reported. Due to resistance patterns, in order to prescribe the right antibiotics, anti-susceptibility testing for each patient before treatment is recommended.
Keywords: https://jmj.jums.ac.ir/article_700339_e91d017746b87311ba76e6c5d6d204f1.pdf