Jahrom University of Medical Sciences Pars Journal of Medical Sciences 2717-0284 20 2 2022 08 23 Frequency of Gene-Producing Strains (aprA, rhlI, rhlR, algD) in Clinical Isolates of Pseudomonas aeruginosa, Isolated from Hospitals of South Fars Frequency of Gene-Producing Strains (aprA, rhlI, rhlR, algD) in Clinical Isolates of Pseudomonas aeruginosa, Isolated from Hospitals of South Fars 39 47 700542 10.22034/pjms.2022.700542 FA Keramat Dorri PhD student, Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran 0000-0002-5802-2216 Farzan Modarresi Department of Bacteriology and Virology, Jahrom Medical School, Jahrom University of Medical Sciences, Jahrom, Iran Mohammadreza Shakibaie Department of Microbiology and Virology, Kerman University of Medical Sciences, Kerman, Iran 0000-0003-2524-125X Elham Moazamian Department of Microbiology, College of Sciences, Agriculture and Modern Technology, Shiraz Branch, Islamic Azad University, Shiraz, Iran 0000-0002-4445-3971 Journal Article 2022 12 25 Introduction: Antibiotic resistance and biofilm production are recognized as two significant factors in the pathogenesis of this bacterium, which are responsible for the long-term persistence of Pseudomonas aeruginosa infections. The present study is designed to examine the antibiotic resistance and the genes involved in the pathogenicity and resistance of this bacterium in the southern region of fars.<br />Material and Methods: The isolates were detected and identified based on standard microbiological and biochemical methods. The antibiotic resistance pattern of Pseudomonas aeruginosa isolates was then determined by antibiotic<br />susceptibility testing (AST) or the antibiogram (disk diffusion) test. The PCR technique was used to detect algD, rhlR, rhlL, and aprA genes.<br />Results: In the study of antibiotic resistance of 40 isolates of Pseudomonas aeruginosa, the effective antibiotics in the treatment of this bacterium were Colistin with 0% resistance Polymyxin B 0%, Meropenem 1 isolate (2.5%), Ciprofloxacin 3 isolates (7.5%), Imipenem 3 isolates (7.5%) and Amikacin 3 isolates (7.5%). In the detection of algD, rhlR, rhlL and aprA genes, 100% of the isolates carried the algD gene, 96% of the isolates carried the rhlR gene, 94% of the isolates carried the rhlL gene and 91% of the isolates carried the aprA gene.<br />Conclusion: The results demonstrated the high presence of algD, rhlR, rhlL, and aprA genes in the Pseudomonas aeruginosa isolates. Considering the high prevalence of the algD gene in the Pseudomonas aeruginosa strains of the current study, which is involved in the capsule production process, it seems essential and vital to control the infections of this bacterium due to the existence of multiple resistance and its ability to produce capsules. Introduction: Antibiotic resistance and biofilm production are recognized as two significant factors in the pathogenesis of this bacterium, which are responsible for the long-term persistence of Pseudomonas aeruginosa infections. The present study is designed to examine the antibiotic resistance and the genes involved in the pathogenicity and resistance of this bacterium in the southern region of fars.<br />Material and Methods: The isolates were detected and identified based on standard microbiological and biochemical methods. The antibiotic resistance pattern of Pseudomonas aeruginosa isolates was then determined by antibiotic<br />susceptibility testing (AST) or the antibiogram (disk diffusion) test. The PCR technique was used to detect algD, rhlR, rhlL, and aprA genes.<br />Results: In the study of antibiotic resistance of 40 isolates of Pseudomonas aeruginosa, the effective antibiotics in the treatment of this bacterium were Colistin with 0% resistance Polymyxin B 0%, Meropenem 1 isolate (2.5%), Ciprofloxacin 3 isolates (7.5%), Imipenem 3 isolates (7.5%) and Amikacin 3 isolates (7.5%). In the detection of algD, rhlR, rhlL and aprA genes, 100% of the isolates carried the algD gene, 96% of the isolates carried the rhlR gene, 94% of the isolates carried the rhlL gene and 91% of the isolates carried the aprA gene.<br />Conclusion: The results demonstrated the high presence of algD, rhlR, rhlL, and aprA genes in the Pseudomonas aeruginosa isolates. Considering the high prevalence of the algD gene in the Pseudomonas aeruginosa strains of the current study, which is involved in the capsule production process, it seems essential and vital to control the infections of this bacterium due to the existence of multiple resistance and its ability to produce capsules. https://jmj.jums.ac.ir/article_700542_1dfddfbb117d06df7631684b7c8bd51e.pdf