Jahrom University of Medical Sciences Pars Journal of Medical Sciences 2717-0284 17 2 2022 12 25 The effect of high-intensity interval training (HIIT) on gene expression of the factors involved in the skeletal muscle metabolism of diabetic rats The effect of high-intensity interval training (HIIT) on gene expression of the factors involved in the skeletal muscle metabolism of diabetic rats 32 39 700416 10.52547/jmj.17.2.32 FA Mona Bostan Manesh Nik Javan Department of Physical Education and Sport Science Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran 0000-0002-6020-9098 Saeedeh Shadmehri Assistant Professor, Department of Physical Education and Sport Science Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran 0000-0002-5000-4427 Mozhgan Ahmadi Assistant Professor, Department of Physical Education and Sport Science Yadegar-e-Imam Khomeini (RAH) Shahre-rey Branch, Islamic Azad University, Tehran, Iran 0000-0002-2431-6953 Journal Article 2022 12 25 Introduction: Improving the metabolism of skeletal muscle is one of the important mechanisms for the treatment of<br />type 2 diabetes. The aim of this study was to evaluate the effect of high-intensity interval training (HIIT) on gene expression of the factors involved in the skeletal muscle metabolism of diabetic rats.<br />Materials and Methods: To implementation of this experimental research, 48 male Wistar rats weighing 220 ± 20 gr randomly were divided into 4 groups including control, diabetes, training and diabetes-training. In this study, the<br />rats become diabetes type 2 using peritoneal injection nicotinamide-STZ. High-intensity interval training performed with intensity of 80-85% VO2max, 5 days a week and for 8 weeks. The expression of GLUT4 and PGC-1α genes were measured by Real Time PCR. Data were analyzed by One-way ANOVA and Tukey post hoc test at the P<0.05.<br />Results: The results showed a significant difference between the mean expression of GLUT4 and PGC-1α of skeletal muscle in diabetic rats in different groups (P< 0.001). The changes in the expression of GLUT4 and PGC-1α of the skeletal muscle in the diabetes group were significantly lower than the control group (P=0.036). High-intensity interval training significantly caused higher the expression of GLUT4 and PGC-1α (P< 0.001).<br />Conclusion: According to the findings of the present study, high-intensity interval training can help to improve cellular energy levels during diabetes as a result of increasing the expression of PGC-1α and GLUT4 in skeletal muscle. Introduction: Improving the metabolism of skeletal muscle is one of the important mechanisms for the treatment of<br />type 2 diabetes. The aim of this study was to evaluate the effect of high-intensity interval training (HIIT) on gene expression of the factors involved in the skeletal muscle metabolism of diabetic rats.<br />Materials and Methods: To implementation of this experimental research, 48 male Wistar rats weighing 220 ± 20 gr randomly were divided into 4 groups including control, diabetes, training and diabetes-training. In this study, the<br />rats become diabetes type 2 using peritoneal injection nicotinamide-STZ. High-intensity interval training performed with intensity of 80-85% VO2max, 5 days a week and for 8 weeks. The expression of GLUT4 and PGC-1α genes were measured by Real Time PCR. Data were analyzed by One-way ANOVA and Tukey post hoc test at the P<0.05.<br />Results: The results showed a significant difference between the mean expression of GLUT4 and PGC-1α of skeletal muscle in diabetic rats in different groups (P< 0.001). The changes in the expression of GLUT4 and PGC-1α of the skeletal muscle in the diabetes group were significantly lower than the control group (P=0.036). High-intensity interval training significantly caused higher the expression of GLUT4 and PGC-1α (P< 0.001).<br />Conclusion: According to the findings of the present study, high-intensity interval training can help to improve cellular energy levels during diabetes as a result of increasing the expression of PGC-1α and GLUT4 in skeletal muscle. https://jmj.jums.ac.ir/article_700416_b614e04d4cb38d93317eb96ad1b8d262.pdf