Jahrom University of Medical Sciences Pars Journal of Medical Sciences 2717-0284 17 2 2022 12 25 Detection of G276T Polymorphism from Adiponectin Gene Using PCR-RFLP Technique in Patients with Breast Cancer Detection of G276T Polymorphism from Adiponectin Gene Using PCR-RFLP Technique in Patients with Breast Cancer 8 14 700413 10.52547/jmj.17.2.8 FA Zahra Tahmasebi Fard Assistant professor of biology group. Islamic Azad of university 0000-0002-9104-6308 Journal Article 2022 12 25 Introduction: Adiponectin hormone is involved in modulating metabolic processes such as glucose regulation and<br />fatty acid catabolism. Several single nucleotide polymorphisms (SNPs) in the transcription region of the gene and its sequences have been identified in different populations that are associated with the prevalence of various diseases. The aim of this study was to determine frequency of G276T polymorphism in breast cancer patients and its comparing in subjects without breast cancer.<br />Materials and Methods: 128 individuals with breast cancer and 128 healthy people were selected after the approval of a specialist physician. After blood collection and DNA extraction, all specimens were amplified with specific primers, then part of them was cut with Mva1269I enzyme and the genotypes of individuals were determined. The results were analyzed using IBM SPSS version 23 software by chi-square and logistic regression tests.<br />Results: The two groups did not have a significant difference for mean age and body mass index. GT genotype was significantly different between the two groups and increased the risk of breast cancer by 1.86 folds in their carriers. None of the genotypes had any relationship with histopathological changes.<br />Conclusion: The T mutant allele at G276T position of adiponectin had a decreasing effect on breast cancer, indicating that this result needs to be investigated in different populations with a larger sample size for confirmation. Introduction: Adiponectin hormone is involved in modulating metabolic processes such as glucose regulation and<br />fatty acid catabolism. Several single nucleotide polymorphisms (SNPs) in the transcription region of the gene and its sequences have been identified in different populations that are associated with the prevalence of various diseases. The aim of this study was to determine frequency of G276T polymorphism in breast cancer patients and its comparing in subjects without breast cancer.<br />Materials and Methods: 128 individuals with breast cancer and 128 healthy people were selected after the approval of a specialist physician. After blood collection and DNA extraction, all specimens were amplified with specific primers, then part of them was cut with Mva1269I enzyme and the genotypes of individuals were determined. The results were analyzed using IBM SPSS version 23 software by chi-square and logistic regression tests.<br />Results: The two groups did not have a significant difference for mean age and body mass index. GT genotype was significantly different between the two groups and increased the risk of breast cancer by 1.86 folds in their carriers. None of the genotypes had any relationship with histopathological changes.<br />Conclusion: The T mutant allele at G276T position of adiponectin had a decreasing effect on breast cancer, indicating that this result needs to be investigated in different populations with a larger sample size for confirmation. https://jmj.jums.ac.ir/article_700413_82142be4970d59df17f51d95b4f363d5.pdf