Jahrom University of Medical Sciences Pars Journal of Medical Sciences 2717-0284 2 2 2022 12 25 Value of Urease Test in the detection of Helicobacter pylori infections Value of Urease Test in the detection of Helicobacter pylori infections 6 11 700319 10.29252/jmj.2.2.3 FA Reza Gottaslou Bahram Kazemi Faransis Megraud Hossein Eliasi Ahad Zargarizadeh Mohammad Rakhshan Hossein Goodarzi Journal Article 2022 12 25 Introduction: H. pylori is microaerophilic, garm negative, spiral organism with 4-6 flagella. The bacterium is an etiologic agent for chronic gastritis and plays a major role in effecting peptic ulcer, gastric cancer, lymphoma. H. pylori infection has a high seroprevalence in Iran (> 90%). Thus diagnosis and  treatment of H.pylori infection is very important. Material and Methods : The presence of H. pylori infection was determined in 100 patients undergoing upper gastrointestinal endoscopy over 9 month period. The presence of H. pyolri was detcted in gastric mucosal biopsy specimens by Biopsy Urease Test, PCR and histopathology. A media was used for urease test, Normal Broth Urea. The biopsy is placed in a broth containing urea and a PH indicator. The goldstandard is histopathology examination. We designed a PCR for amplifying the H. pylori gene encoding ure C (294 bp). Results : The results of urease test were 66.6% sensitivity and 69.7% specefity. The results of PCR assay were 98.6% sensitivity and 93% specifity. There was a significant difference between the specifity and sensitivity of urease test and PCR. Conclusion : The result of This study showed  that urease test was not a sensitive and specific method for accurate detection of  the infection. It a level of the sensitivity is too low to be used as the sole method of detection in ordinary practic. In this situation other/ tests should be used. Introduction: H. pylori is microaerophilic, garm negative, spiral organism with 4-6 flagella. The bacterium is an etiologic agent for chronic gastritis and plays a major role in effecting peptic ulcer, gastric cancer, lymphoma. H. pylori infection has a high seroprevalence in Iran (> 90%). Thus diagnosis and  treatment of H.pylori infection is very important. Material and Methods : The presence of H. pylori infection was determined in 100 patients undergoing upper gastrointestinal endoscopy over 9 month period. The presence of H. pyolri was detcted in gastric mucosal biopsy specimens by Biopsy Urease Test, PCR and histopathology. A media was used for urease test, Normal Broth Urea. The biopsy is placed in a broth containing urea and a PH indicator. The goldstandard is histopathology examination. We designed a PCR for amplifying the H. pylori gene encoding ure C (294 bp). Results : The results of urease test were 66.6% sensitivity and 69.7% specefity. The results of PCR assay were 98.6% sensitivity and 93% specifity. There was a significant difference between the specifity and sensitivity of urease test and PCR. Conclusion : The result of This study showed  that urease test was not a sensitive and specific method for accurate detection of  the infection. It a level of the sensitivity is too low to be used as the sole method of detection in ordinary practic. In this situation other/ tests should be used. https://jmj.jums.ac.ir/article_700319_014e106a09c70898e58abf01a62a0a80.pdf