:: Volume 15, Issue 1 (spring 2017) ::
jmj 2017, 15(1): 36-42 Back to browse issues page
Evaluating the ability of Biofilm formation in Escherichia coli isolated from clinical samples in Zahedan
Sara Jalilian, Farrokh Rokh Bakhsh zamin
Abstract:   (2771 Views)

Introduction:

Escherichia coli (E.coli) is the main causative pathogen in urinary tract infections (UTIs). Antibiotic resistance based on Extended Spectrum -Lactamase (ESBL) producing Escherichia coli strains is reported to be the cause of community and hospital acquired infections therefore this this is an important problem in public health. The aim of this study was to identify the blaTEM, blaSHV and CTX genes in clinical isolates of E. coli recovered from patients with UTIs in Zahedan, Iran.

Methods and Materials:

Escherichia coli isolates (N=60) were analyzed by biochemistry and phenotypic methods. Each of the initial E. coli screening test isolate was investigated for the presence of blaTEM, blaSHV and blaCTX-M genes via polymerase chain reaction (PCR) using gene-specific primers.

Results:

Sixty tested isolates; the prevalence of blaTEM and blaCTX-M genes was determined to be 68.3%, 46.6% but blaSHV gene was not detected. Based on phenotypic tests, biofilm capacity was detected in 10 clinical isolates.

Conclusions:

The results of this study show that the use of adequate doses of antibiotics in the treatment of infections may be caused by biofilms are emphasized. Rapid detection of biofilm formation, therefore isolates have the ability to decide care and management is necessary.

Keywords: Escherichia coli, antibiotic resistance, PCR, biofilm
Full-Text [PDF 274 kb]   (634 Downloads)    
Type of Study: Research | Subject: Microbshensi
Received: 2017/04/22 | Accepted: 2017/07/4 | Published: 2017/07/5



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Volume 15, Issue 1 (spring 2017) Back to browse issues page