:: Volume 17, Issue 2 (summer 2019) ::
jmj 2019, 17(2): 8-14 Back to browse issues page
Detection of G276T Polymorphism from Adiponectin Gene Using PCR-RFLP Technique in Patients with Breast Cancer
Zahra Tahmasebi Fard Dr
Islamic Azad of university
Abstract:   (833 Views)
Adiponectin hormone is involved in modulating metabolic processes such as glucose regulation and fatty acid catabolism. Several single nucleotide polymorphisms (SNPs) in the transcription region of the gene and its sequences have been identified in different populations that are associated with the prevalence of various diseases. In this study, PCR-RFLP technique was used to identify G276T polymorphism in patients with breast cancer.
Material and Methods:
128 individuals with breast cancer and 128 healthy people were selected after the approval of a specialist physician. After blood collection and DNA extraction, all specimens were amplified with specific primers, then part of them was cut with Mva1269I enzyme and the genotypes of individuals were determined. The results were analyzed using SPSS version 23 software by chi-square and logistic regression tests.
The two groups did not have a significant difference for mean age and body mass index. TT and GT genotypes were significantly different in the two groups and increased the risk of breast cancer by 2.987 and 1.489 in their carriers, respectively. GG and GT genotypes showed a significant relationship with age and body mass index.
The results showed a strong association between adiponectin G276T polymorphism and breast cancer. Carriers of the heterozygote GT genotype were more likely to develop breast cancer with increasing age and body mass index.
Keywords: Breast Cancer, Polymorphism, Adiponectin, RFLP (Restriction Fragment Length Polymorphisms)
Full-Text [PDF 530 kb]   (138 Downloads)    
Type of Study: Research | Subject: ect
Received: 2018/12/4 | Accepted: 2019/09/11 | Published: 2019/10/20

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Volume 17, Issue 2 (summer 2019) Back to browse issues page