:: Volume 16, Issue 3 (fall 2018) ::
jmj 2018, 16(3): 10-16 Back to browse issues page
Identification of β-lactamase genes in Escherichia coli isolated from diabetic foot ulcer by Multiplex PCR in Jahrom city
Mojtaba Akrami , Zahra Karga jahromi , Zhila Rahmanian , Hoda Haghshenas
Jahrom University of Medical Sciences
Abstract:   (886 Views)
Diabetic foot ulcers, which are complications of diabetes mellitus, can be accompanied by an infection in the wound, which can increase the risk of amputation or even death. E coli bacteria, which have been reported to be present in diabetic foot ulcers, can lead to worsening of the condition of the wound. The aim of this study was to detect β-lactamase genes in Escherichia coli isolated from diabetic foot ulcer by Multiplex PCR in Jahrom city.
method:
Determination of antibacterial sensitivity of Escherichia coli isolated using disc diffusion method. The detection of strains producing ESBL enzymes was performed using Double disk synergy test. In order to detect carbapenem resistant organisms in the TSF, the concentration of 0.5 McFarland was used. To isolate and identify MBL-producing strains, the Etest MBL (AB BioDisk, Solna, Sweden) tapes was used according to the manufacturer's instructions. In a multiplex PCR assay, using specific primers, the presence of genes encoding beta-lactamase enzymes including blaTEM, blaSHV and blaOXA-1 was studied.
Keywords: Diabetic foot ulcer, E coli, Antibiotic resistance, Beta-lactamase
Full-Text [PDF 759 kb]   (148 Downloads)    
Type of Study: Research | Subject: Microbshensi
Received: 2018/04/22 | Accepted: 2018/09/12 | Published: 2018/10/13


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Volume 16, Issue 3 (fall 2018) Back to browse issues page