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:: Volume 14, Number 3 (fall 2016) ::
jjums 2016, 14(3): 35-42 Back to browse issues page
Isolation and culture of mouse newborn skin fibroblast cells in a simple and inexpensive method
Alireza Kheirollah, Mostafa Chashmpoosh, Asma Mohammadi, Neda Abdovis, Hamide Ghasempoor
Student Department of Biochemistry, Faculty of Medical Sciences, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Ira
Abstract:   (1103 Views)

Background  and  objective

Cell culture is one of the main techniques used in scientific researches that done in three ways: Primary culture, secondary culture or cell passage and purchase Cell Lines of Cell banks. Fibroblast cell is an important component of connective tissue that is found almost everywhere in the body. Fibroblast cells based on their location have functional and morphology roles. The aim of this project was simplification through innovation and localization, and to obtain mouse newborn skin fibroblast cells in order to use these cells for further study.

Methods

For this project were used from mouse neonatal that is one to two days. Initially all devices, including surgical materials, culture dishes, buffer and etc were sterilized by autoclaving at 121 ° C and intense pressure. As well as workplace were sterilized by 70% alcohol and UV. Initially mouse neonatal under the biology hood and in 70% alcohol were killed and were sterilized with sterile instruments and then skin fibroblast cells were isolated from these mice. In addition skin fibroblast cells for further growth were transferred of the primary environment to a fresh and new medium and first passages was performed on these cells.

Results

Fibroblast cells in different days were evaluated in terms of growth and morphology by fluorescent microscope (Olympus) with a magnification of 10, that this cells in terms of morphology were appropriate.

Conclusion

According to the results of this study, the simplicity of this method, no need to high costs and sterile conditions without the use of antibiotics and anti-fungal, makes reasonable use of this method for isolation and cell culture in different studies.

Keywords: Cell culture, Fibroblast cells, Mouse newborn

Keywords: Cell culture, Fibroblast cells, Mouse newborn
Full-Text [PDF 555 kb]   (251 Downloads)    
Type of Study: Applicable | Subject: ect
Received: 2016/03/29 | Accepted: 2016/09/6 | Published: 2017/03/8
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Kheirollah A, Chashmpoosh M, Mohammadi A, Abdovis N, Ghasempoor H. Isolation and culture of mouse newborn skin fibroblast cells in a simple and inexpensive method. jjums. 2016; 14 (3) :35-42
URL: http://jmj.jums.ac.ir/article-1-682-en.html
Volume 14, Number 3 (fall 2016) Back to browse issues page
فصلنامه دانشگاه علوم پزشکی جهرم Journal of Jahrom University of Medical Sciences
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