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:: Volume 14, Number 3 (fall 2016) ::
jjums 2016, 14(3): 8-17 Back to browse issues page
The frequency of qacA/B and smr genes in clinical isolates of methicillin resistance coagulase negative staphylococci
mohammad bokaeian, Javad Adabi, Hamed Tahmasebi [ORCID]
MSc of microbiology Zahedan University of medical science
Abstract:   (821 Views)

Introduction:

 Increasing use of disinfectants biocide cause to appearance of resistant strains of coagulase negative Staphylococcus. Some research confirmed this gene responsible for resistance to methicillin and association with these agents. The aim of this study was to investigate the presence of resistance genes of biocides such as qac A/B and smr in coagulase negative staphylococci.

Materials & Methods:

In this cross-sectional study, 60 samples of Staphylococcus epidermidis and 49 samples of Staphylococcus saprophyticus collected over a period of 9 months from clinical samples. After the initial biochemical tests and confirmed genus and species isolates, using specific primers for aqcA / B and smr genes studied by using polymerase chain reaction(PCR) method. Data were analyzed using chi-square test

Results:

 Of 60 isolates of Staphylococcus saprophyticus, 36 isolates had mecA gene. Among these, significantly 19 isolates qacA gen and 21 isolated smr gene. also of 49 Staphylococcus epidermidis isolates, 27 isolates were mecA gene and among those isolates ,11 isolates have gene qacA / B and 8 isolate have smr genes.

Conclusions:

The results of this study, we observed the wide spread presence of genes qac A/B and smr in clinical isolates of methicillin-resistant coagulase-negative staphylococci. Due to the prevalence methicillin-resistant strains obtained in isolation,

Keywords: qacA/B, smr, mecA, methicillin resistance, coagulase negative
Full-Text [PDF 473 kb]   (162 Downloads)    
Type of Study: Research | Subject: Microbshensi
Received: 2016/07/15 | Accepted: 2016/11/8 | Published: 2017/03/13
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bokaeian M, Adabi J, Tahmasebi H. The frequency of qacA/B and smr genes in clinical isolates of methicillin resistance coagulase negative staphylococci . jjums. 2016; 14 (3) :8-17
URL: http://jmj.jums.ac.ir/article-1-840-en.html
Volume 14, Number 3 (fall 2016) Back to browse issues page
فصلنامه دانشگاه علوم پزشکی جهرم Journal of Jahrom University of Medical Sciences
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