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:: Volume 19, Issue 1 (spring 2021) ::
jmj 2021, 19(1): 34-43 Back to browse issues page
Determination of Physicochemical Properties of Human Immunoglobulin G- Fc Fragment by Bioinformatic
Soheyla Rohani , Fatemeh Hajighasemi *
Shahed University
Abstract:   (683 Views)
Introduction: Immunoglobulins (Igs) are defensive glycoproteins specifically recognize and destroy pathogens. Immunoglobulin G (IgG) is most abundant Ig in serum has important protective role against infections. Fragment of crystallizable (Fc) in IgG has essential role in pathogens destruction. Determination the physicochemical properties of IgG Fc is useful in well recognition of its function, diagnostic and therapeutic purposes. Bioinformatic is an efficient scientific field uses abundant biological information collected in computers for solving biologic problems. Aim of this study is recognition physicochemical features of human IgG Fc by bioinformatic.
Materials and Methods: Amino acid sequence and third structure of reference human IgG were found in PDB (Protein Data Bank)  database. Second IgG structure was determined by Protein Homology/analogY Recognition Engine V 2 (Phyre 2) software. Physicochemical properties (felexibility, accessibility and hydrophilicity) of IgG Fc fragment were identified by IEDB (Immune Epitope Database) software.
Results: According to results of this study, most accessibe, hydrophilic and felexible sites of IgG Fc fragment were located to 200 – 450 amino acid sequences. Moreover most accessibe, hydrophilic and felexible positions were overlapped in 291- 300 and 381- 405 amino acid sequences and could be most probable locations of epitopes.
Conclusion: Physicochemical properties of IgG Fc fragment identified in present study are valuable in more exact recognition of IgG functions and its immunogenic epitopes which could be helpful in producing of specific monoclonal anti IgG antibodies for production IgG diagnostic tools, optimizing existing kits, development of similar proteins for diagnostic and therapeutic purposes and phylogenic studies.
Keywords: IgG, Bioinformatic, Physicochemical
Full-Text [PDF 1031 kb]   (158 Downloads)    
Type of Study: Research | Subject: Imnology
Received: 2020/12/19 | Revised: 2021/05/31 | Accepted: 2021/05/5
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Research code: 524
Ethics code: IR.Shahed.REC.524

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Rohani S, Hajighasemi F. Determination of Physicochemical Properties of Human Immunoglobulin G- Fc Fragment by Bioinformatic. jmj. 2021; 19 (1) :34-43
URL: http://jmj.jums.ac.ir/article-1-1560-en.html

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Volume 19, Issue 1 (spring 2021) Back to browse issues page
فصلنامه دانشگاه علوم پزشکی جهرم Journal of Jahrom University of Medical Sciences
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