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:: Volume 17, Issue 1 (spring 2019) ::
jmj 2019, 17(1): 49-54 Back to browse issues page
Frequency of bacterial contamination of platelet products in blood transfusion organization of Jahrom in 2016-17
Akbar Kazemi , Fatemeh Atashi , Reyhneh Rouhi jahromi , Danesh Javeshghani , Pejman Hamedi-Asl , Fatemeh Vahidinezhad , Abdolrahman Mohtashemifar , Zahra Hooshyar , Zahra Rezaee , Nazila Shiravani , Pegah Eidizadeh , Farnoosh Hatami
Jahrom University of Medical Science
Abstract:   (321 Views)
Abstract
Background and objective
Most cases of blood transfusion-associated bacterial sepsis are caused by platelet concentrates (PCs). Screening of PCs is carried out in the Iranian quality control plans as it is essential for detection of bacterial contamination. With regard to quality control, this study investigated the frequency of bacterial contamination of PCs through Gram staining and microbial culture methods.
Materials and methods
In this descriptive cross-sectional study, 880 cords out of 440 platelet product bags (two cords per platelet bag) were selected on the first and third days and included in the study. Direct smears were prepared from the samples for Gram staining, then they were cultured in blood agar and chocolate agar under aerobic and microaerophilic conditions for detection of both Gram positive and negative cocci and Gram positive bacteria. Cultures were also made in MacConkey medium under aerobic conditions to identify Gram negative bacilli. In cases of bacterial growth and colony formation, differential tests were performed to detect the bacteria.
Findings
One case of Staphylococcus saprophyticus was isolated of 440 platelet bags collected from donors in Jahrom Blood Transfusion Organization, from which two platelet cord (880 samples) had been isolated on the first and third days. Accordingly, a contamination frequency of 0.23% was detected for platelet products in Jahrom city.
Conclusion
Performing staining and bacterial culture from all of the platelet cords before they are used is a good way to ensure safety and prevent bacterial contamination from platelet products and quality control.
 
Keywords: Platelet, bacterial contamination, bacterial culture, blood transfusion, blood donors
     
Type of Study: Research | Subject: Microbshensi
Received: 2018/12/17 | Accepted: 2019/06/22 | Published: 2019/09/11
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Kazemi A, Atashi F, Rouhi jahromi R, Javeshghani D, Hamedi-Asl P, Vahidinezhad F, et al . Frequency of bacterial contamination of platelet products in blood transfusion organization of Jahrom in 2016-17. jmj. 2019; 17 (1) :49-54
URL: http://jmj.jums.ac.ir/article-1-1265-en.html


Volume 17, Issue 1 (spring 2019) Back to browse issues page
فصلنامه دانشگاه علوم پزشکی جهرم Journal of Jahrom University of Medical Sciences
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