مجله علوم پزشکی پارس

ژن مقاوم به متی سیلین و موپیروسین در استافیلوکوکوس اورئوس جدا شده از پرسنل بیمارستان شهرستان داراب با روش Multiplex PCR

نویسنده

بخش میکروبشناسی،دانشگاه آزاد اسلامی جهرم، جهرم، ایران

چکیده

مقدمه: استافیلوکوکوس اورئوس مقاوم به متی سیلین  (MRSA ) Methicillin-Resistant Staphylococcus aureus و موپیروسین از پاتوژن های مهم بیمارستانی می باشد. یکی از منابع مهم این ارگانیسم در عفونت بیمارستانی، پرسنل بیمارستان می باشند. هدف این تحقیق تعیین فراوانی ژن مقاوم به متی سیلین و موپیروسین در استافیلوکوکوس اورئوس جدا شده از پرسنل بیمارستان با روش Multiplex PCR می باشد.
روش کار: در این مطالعه 150 نفر از پرسنل بیمارستان بررسی گردیدند و نمونه استافیلوکوکوس اورئوس جدا شده از آنها با روش های بیوشیمیایی و میکروبی تایید شدند. جهت تعیین میزان حساسیت سویه ها نسبت به آنتی بیوتیک متی سیلین و موپیروسین از روش دیسک دیفیوژن استفاده شد. سپس، حضور ژن های mup ،  mec A  و  nuc با استفاده از Multiplex PCR بررسی شد.
یافته ها: از بین 150 فرد مورد مطالعه، 120 نفر (80%) زن بودند. در مجموع 53 نفر (35.3%) ناقل استافیلوکوکوس اورئوس بودند که 42 نفر زن و 11 نفر مرد بودند. از 11 ایزوله جدا شده از مردان، 4 مورد مقاوم به متی سیلین بودند و هیچ کدام به موپیروسین مقاوم نبودند. از 42 ایزوله جدا شده از زنان، 11 مورد مقاوم به متی سیلین و 2 مورد مقاوم به موپیروسین بودند. . با استفاده از روش مولکولی، تعداد 11 ایزوله (9.5%) دارای ژن mecA بودند و هیچ سویه ای دارای ژن mup  نبودند.
نتیجه گیری: شیوع استافیلوکوکوس اورئوس مقاوم به متی سیلین و موپیروسین میان پرسنل بیمارستان داراب، نسبتاً کم بود. با این حال کنترل دائمی ناقلین و درمان آنها می تواند از اشاعه این باکتری و عفونت های حاصل از آن جلوگیری نماید.
 

کلیدواژه‌ها

عنوان مقاله [English]

Methicillin and Mupirocin resistance genes in Staphylococcus aureus isolated from hospital staff in Darab by using of Multiplex PCR

نویسنده [English]

  • Houshnag Jamali

Department of Microbiology, Jahrom Branch, Islamic Azad University, Jahrom, Iran

چکیده [English]

Introduction:
Methicillin and Mupirocin –resistance Staphylococcus aureus (MRSA) are considered as the important hospital pathogens. One of the important resources of this organism in nosocomial infection is related to hospital personnel. The objective of this research was is to investigate the frequency of MRSA gene in staphylococcus aureus isolated from Hospital staff in Darab by multiplex PCR.
Materials and Methods: In this study, 150 hospital staffs were investigated and specimens of staphylococcus aureus which isolated from those were confirmed by biochemical and microbial methods. The disk diffusion method
was used to determine the susceptibility of the isolates to methicillin and mupirocin antibiotic. Then, the presence of mec-A, mup and nuc genes were investigated by using of Multiplex PCR method.
Results: Among of 150 subjects investigated, 120 ones (80%) were female. Totally, 53 people (35.3%) were carriers of Staphylococcus aureus which 42 and 11 were female and male, respectively. Among of the 11 staphylococcus aureus isolated in men, 4 cases were methicillin-resistant and any of them were not mupirocin-resistant. Of the 42 staphylococcus aureus isolated in women studied, 11 cases were methicillin-resistant and 2 cases were mupirocin-resistant. Using of molecular method, 11 isolates (9.5%) were contained mec-A gene and any of isolates were not contained mup gen.
Conclusion: The frequency of methicillin- and mupirocin-resistant staphylococcus aureus among the personnel of
Darab Hospital was relatively low. However, permanent control of the carriers and treatment of them can prevent the spread of this bacterium and its associated infections.

کلیدواژه‌ها [English]

  • Staphylococcus Aureus
  • Mupirocin
  • Methicillin
  • Multiplex PCR
  • Antibiotic Resistance
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مجله علوم پزشکی پارس
دوره 17، شماره 3 - شماره پیاپی 49
مهر 1398
صفحه 35-42